Preparation and Lysozyme Adsorption Performance of Meso-structured Cellular Foams with Different Pore Size
Abstract: The mesostructured cellular foams (MCFs) were synthesized using microemulsion templating, in which the nonionic triblock copolymer surfactant Pluronic P123 was served as template and 1,3,5-trimethylbenzene (TMB) as organic swelling agent. By controlling the mass ratio of TMB/P123, a series of MCFs with different pore size were prepared, and then the structural and chemical properties of MCFs were characterized by TEM and nitrogen adsorption. It was found that the pore size of MCFs increased when the mass ratio of TMB/P123 increased from 0.5 to 1.0, while that of MCFs decreased when the mass ratio continuously increased to 1.5. The MCFs were used as adsorbent for the adsorption of lysozyme. The maximum adsorption rate was obtained on MCFs-1.5 (mass ratio of TMB/P123 was 1.5), meaning that the sample reached equilibrium within 1 h. The immobilization of lysozyme on MCFs-1.5 prevented the leaching of enzyme effectively and the immobilized amount was up to 490 mg/g. Furthermore, FT-IR analysis revealed that the lysozyme adsorbed on MCFs could be held without evident structure changes. This study suggested that enzyme loading efficiency was clearly dependent on the size matching between the enzyme molecules and carrier pores, and the synthesized MCFs could be applied as excellent carriers for enzyme immobilization.
文章引用: 李 俊 , 尹光福 , 丁 艺 (2013) 不同孔径MCFs的制备及其对溶菌酶的吸附性能。 材料科学， 3， 56-60. doi: 10.12677/MS.2013.32011
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