口腔幽门螺杆菌尿素酶的检测及其临床意义
The Clinical Value of Oral H. Pylori Antigen Test

作者: 叶国钦 :螺旋杆菌研究所,西雅图,美国;

关键词: 幽门螺杆菌检测方法HPSH. Pylori Diagnosis Method HPS

摘要:
幽门螺杆菌(Hp)感染诊断方法很多,各种诊断方法有其各自的特点,但传统Hp感染的诊断方法,主要适用于胃Hp感染的检测,对口腔Hp感染难以做出稳定的检测结果,甚至存在检测盲区。唾液幽门螺杆菌抗原测试板(HPS),是应用胶体金层析式双抗体夹心法技术,采用Hp尿素酶单克隆抗体,定性检测人体唾液中相应Hp产生的尿素酶,主要应用于口腔Hp感染的检测,具有较高的敏感性和特异性,操作简便、快捷,可在实验室外进行检测等特点。HPSUBT联合检测,可大致区分口腔Hp感染合并胃Hp感染、单纯口腔Hp感染、单纯胃Hp感染,为临床制定更完善的、全新的Hp根除方案提供实验诊断依据。
There are number of diagnostic methods detecting Helicobacter pylori (H. pylori) infection. Each has their own character, however standard diagnosis technology apply in detecting H. pylori infection of stomach, not suitable for diagnosis of oral infection. For example, C13, C14 UBT test had been utilized as a gold standard for diagnosis of H. pylori infection in stomach more than two decades, however they have blinded zone detecting oral H. pylori infection. A simple noninvasive, new technology, H. pylori saliva antigen test (HPS) to detecting oral infection with a clean view of the line linking oral and stomach H. pylori infection. HPS is a sandwich immunoassay test, which utilizes an unique antibodies to selectively identify H. pylori Urease in saliva. The HPS technology has high sensitivity and specificity. It is very simple procedure of test and the results can be obtained within few minutes. It is suitable for lab use and over the counter. The combination uses of HPS and UBT can classify both oral and stomach infection, or simple oral infection or simple stomach infection. By this technology we can make an revolutionary treatment plan for cure H. pylori infection.

文章引用: 叶国钦 (2012) 口腔幽门螺杆菌尿素酶的检测及其临床意义。 医学诊断, 2, 23-29. doi: 10.12677/MD.2012.24005

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