农杆菌介导DR1372基因转化甘蓝型油菜
Preliminary Study on Agrobacterium-Mediated Transformation of DR1372 Gene into Brassica napus L.

作者: 张思维 , 周文波 , 张 新 , 陈翠娜 , 代其林 :西南科技大学生命科学与工程学院,绵阳; 王 劲 :中国农业科学院生物技术研究所,北京;

关键词: DR1372油菜农杆菌介导遗传转化 DR1372 Brassica napus L. Genetic Transformation Drought-Tolerance

摘要:

DR1372基因是在耐辐射奇球菌(Deinococcus radiodurans, DR)基因组中克隆得到的一个基因,其蛋白序列存在一个WHy功能域,此功能域可能参与油菜的抗旱过程。本研究首先构建植物DR1372- pBI121表达载体,然后利用农杆菌介导法将目的基因DR1372成功转入甘蓝型油菜中,并对影响油菜再生频率以及遗传转化效率的几个因素进行了探讨,初步建立了油菜转DR1372基因遗传转化体系,为DR1372基因的功能研究奠定了基础。

Abstract: The DR1372 containing a special domain called WHy was cloned from Deinococcus radiodurans. This WHy domain may be involved in the mechanism of drought resistance. In this study, the plant expression vector DR1372-pBI121 was constructed and introduced into Brassica napus L. mediated by Agrobacteria. Several factors affecting genetic transformation efficiency were explored and the Agrobacterium-mediated genetic transformation system was set up in Brassica napus. This study provides essential materials for later research on DR1372 gene.

文章引用: 张思维 , 周文波 , 张 新 , 陈翠娜 , 代其林 , 王 劲 (2013) 农杆菌介导DR1372基因转化甘蓝型油菜。 植物学研究, 2, 40-45. doi: 10.12677/BR.2013.21007

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