Isolation of Sperm Cells of Jatropha curcas L.
Abstract: Mature pollen of Jatropha curcas L. was broken in solutions with different osmolalities, and re-leased its cytoplasmic content, including two sperms. The isolated sperms could keep viability fluorescence for 20 - 30 min and were collected to concentration using a micromanipulator. The state of isolated sperms in different osmolalities was analyzed. Optimal concentration of isolation solution was compared according to the isolated sperm number and its duration of keeping viability and 7.5% glucose was best for isolating the sperm cells. The sperm isolation of Jatropha curcas L. makes a basis for its reproduction engineering study and provides study material to research its sperm development.
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