Isolation of Salt-Inducible Promoter in Maize and Construction of Plant Expression Vector
Abstract: The upstream nucleotide sequence of maize salt-inducible protein kinase, named STY, was isolated from the genomic DNA of maize by PCR. Promoter sequence analysis by PlantCARE showed that it had some typical cis-elements, including TATA box and CAAT box. In addition, there were two salt responsive elements GT1-motif, various phytohormone responsive elements and stress responsive elements in the promoter sequence. The fusion gene of STY promoter and GUS were constructed and named pCAM-STY. The vector was trans-formed into Agrobacterium tumefaciens. The vector containing STY promoter was transferred into tobacco by Agrobacterium tumefaciens mediated method, function of STY promoter was analyzed by identifying activation of GUS using histochemistry staining. The results indicated that the promoter activated expression of GUS re-port gene, It laid the foundation to resolve the serious multi-stress problems of agriculture in northeast of China.
文章引用: 郑亚杰 , 冯頔 , 魏毅 , 刘金亮 , 贾保磊 , 张艳华 , 潘洪玉 , 张世宏 (2012) 玉米盐诱导型启动子克隆及其植物表达载体构建。 植物学研究， 1， 30-35. doi: 10.12677/br.2012.12005
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