镧对铽荧光增强效应测定药物加替沙星胶囊中的加替沙星含量
Determination the Gatifloxacin Drug Content in Gatifloxacin Capsule by Effect of Lanthanum on Terbium Fluorescence Enhancement Effect

作者: 赵悦辉 , 李烨 , 李刚 , 宋冬雪 * , AbdullahAbdu Ahmed , 郑极慧 , 吕玉光 :佳木斯大学药学院; 谭博 , 马丽丽 :佳木斯市食品药品检验所; 王冠乔 :沈阳药科大学;

关键词: 稀土离子喹诺酮类加替沙星荧光分光光度法Rare Earth Ions Quinolone Gatifloxacin Fluorescence Spectrophotometry

摘要:
探讨第四代喹诺酮类药物加替沙星与稀土离子铽离子形成的配合物的荧光特性。应用荧光分光光度计测定药物加替沙星与稀土离子铽离子形成的配合物的荧光强度,通过加入表面活性剂、加入共发光离子La3+、改变溶液的pH等增敏方法选择最佳条件。镧对该体系具有较强的荧光增强效应,最佳的pH值为7.5,选用0.1 mol·L–1 NH4Cl-HCl效果最佳,加替沙星的最佳浓度为1.0 × 10–6 mol·L–1。对荧光新体系的荧光增强作用的机理进行了初步探讨,最终建立了Tb3+-La3+-GFLX-SDS新体系,上述研究对于稀土元素分析、生物和药物分析都具有潜在的应用价值,为稀土和喹诺酮类药物配合物的相关研究提供了一定的理论和实验基础,可以用于测定人工合成样品和制剂的含量,该方法选择性好,灵敏度高,结果令人满意。

Abstract:
To study fluorescence of the forth generation of quinolones gatifloxacin and the rare earth ion complexes. Apply fluorescence spectrophotometry detects the fluorescence intensity of gatifloxacin and the rare earth ion com-plexes. Through the surface active agent, change pH and the emitting ions were added to enhance the fluorescence in-tensity to achieve the establishment of a high sensitivity, high selectivity of the fluorescent system. Tb3+-GFLX-SDS system can be strongly emitted by La3+, the best pH 7.5, GFLX 1.0 × 10–6 mol·L–1, 0.1 mol·L–1 NH4Cl-HCl. The fluo-rescence enhancement mechanism was discussed. Tb3+-La3+-GFLX-SDS new system was established. Rare earth ele-ment analysis for the study, biological and pharmaceutical analysis has potential applications for rare earth complexes of a quinolone-related research provide a theoretical and experimental basis. A test method based on this co-lumines- cence system was applied to the determination of the medicine content with satisfactory results.

文章引用: 赵悦辉 , 李烨 , 谭博 , 马丽丽 , 王冠乔 , 李刚 , 宋冬雪 , AbdullahAbdu Ahmed , 郑极慧 , 吕玉光 (2012) 镧对铽荧光增强效应测定药物加替沙星胶囊中的加替沙星含量。 药物资讯, 1, 15-20. doi: 10.12677/PI.2012.12004

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