“绿岭”核桃种胚组培苗腋芽快繁体系的初步研究
Rapid Propagation of “Lv Ling” Walnut (Juglans Regia L.) through Embryo-Induced Seedlings

作者: 刘喜星 , 李保国 , 齐国辉 , 回彦哲 , 董丽欣 , 马丽媛 :河北农业大学林学院; 顾玉红 , 刘 璐 :河北农业大学生命科学学院;

关键词: 核桃核桃属腋芽激素 Walnut Juglans Embryo Axillary Buds Hormones

摘要:

以早实薄皮核桃绿岭成熟种胚诱导出的腋芽为外植体,MS为基本培养基,通过调节激素种类和浓度配比,初步研究了“绿岭”核桃种胚组培苗腋芽快繁体系。比较试验结果表明:最佳种胚萌发培养基为MS + 蔗糖30 g/L + 琼脂6 g/L;最佳腋芽诱导和增殖培养基为MS + 6-BA 2.0mg/L + NAA 0.2 mg/L + 蔗糖30 g/L + 琼脂6 g/L。生根采用两步生根法:先在DKW + IBA 8.0 mg/L + 蔗糖30 g/L + 琼脂6.0 g/L暗培养20 d,然后转入DKW + 蔗糖30 g/L + 琼脂6 g/L培养基中进行光照培养,生根率为50%。诱导生根的组培苗移栽到大田后成活率为33.3%

Abstract:

The mature embryo induced axillary buds were used as explants to investigate rapid propagation of precocious thin-shelled “Lv Ling” walnut in the MS basic medium with different concentrations of plant growth regulators. The results showed that MS + sucrose 30 g/L + agar6 g/L was the most feasible medium for embryo germination, and MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L + sucrose30 g/L + agar6 g/L was the best for induction and multiplication of the axillary buds in all tested treatments. The 50% of rooting rate was achieved by a two-stage rooting method, namely, the shoots were first cultivated in DKW + IBA 8.0 mg/L + sucrose30 g/L + agar6.0 g/L medium for 20 days under dark condition, then transferred to DKW+ sucrose30 g/L + agar6.0 g/L medium under light condition. After transplanting to the field, 33.3% of the seedlings survived.

 

文章引用: 刘喜星 , 顾玉红 , 李保国 , 齐国辉 , 刘 璐 , 回彦哲 , 董丽欣 , 马丽媛 (2012) “绿岭”核桃种胚组培苗腋芽快繁体系的初步研究。 农业科学, 2, 25-29. doi: 10.12677/hjas.2012.22005

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