长春花细胞生物碱合成培养过程的营养消耗研究
The Study of Nutrient Consumption on Catharanthus Roseus Cell in the Alkaloid Biosynthesis Culture

作者: 赵 楠 , 陈 文 , 郭志刚 :清华大学化学工程系,北京 ;

关键词: 长春花细胞培养生物反应器萜类吲哚生物碱长春碱Catharanthus Roseus Cell Culture Bio-Fermenter Terpene Indole Alkanoid Vinblastine

摘要: 本文利用EPC-500PE型植物细胞反应器培养了长春花细胞,筛选到较优的参数组合为150 r.p.m搅拌速度和0.5 mL/min供气量。在合成培养过程中长春花细胞的蔗糖消耗量约为初始添加浓度(30 g/L)50%,硝态氮的消耗量为初始添加浓度的90%,氨态氮的消耗量为初始添加浓度的50%,磷的消耗量为初始添加浓度的70%。长春碱含量在第5天达到峰值。

Abstract: Catharanthus roseus cells were cultured using the EPC-500 PE bio-fermenter, and a stirring speed of 150 r/min and gas supply of 0.5 ml/min were determined to be the best cultivating conditions. During the cultivation process, the cells’ sucrose consumption was about 50% of the initial addition (30 g/L), while nitrate consumption, ammonia nitrogen consumption and phosphorus consumption were 90%, 50% and 70% of the initial addition, respectively. The content of vinblastine peaked at the fifth day.

文章引用: 赵 楠 , 陈 文 , 郭志刚 (2012) 长春花细胞生物碱合成培养过程的营养消耗研究。 生物过程, 2, 58-64. doi: 10.12677/bp.2012.22010

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