药用真菌杨树菇SUMO基因及抗肿瘤活性初步鉴定
The Molecular Cloning and Antitumor Activity Characterization of SUMO from Medicinal Fungus Agrocybe aegerita

作者: 梁一 :广东医学院检验学院临床免疫教研室,东莞; 白娟 :河南中医学院,郑州; 孙慧 :武汉大学生命科学学院,武汉;

关键词: 药用真菌SUMO抗肿瘤凋亡<br>Medicinal Fungus SUMO Antitumor Activity Apoptosis

摘要:
目的:克隆杨树菇抗肿瘤相关蛋白基因,初步鉴定其活性。方法:构建cDNA文库,随机测序得到SUMO (Small Ubiquitin-like Modifier)基因,通过构建真核表达质粒,并检测其在HeLa细胞中表达引起的细胞凋亡及衰老的变化,初步鉴定其抗肿瘤活性。结果:得到全长为606 bp的SUMO cDNA序列,编码103个氨基酸,将表达SUMO的真核表达质粒转染入HeLa细胞中,发现可引起肿瘤细胞的凋亡,但对细胞衰老无影响。结论:药用真菌中SUMO等小泛素类蛋白酶系统值得进一步研究,有助于新型抗肿瘤药物前体的开发。
Objective: The antitumor-associated gene of Agrocybe aegerita was cloned to identify the bioactivity. Method: cDNA library was constructed and the gene SUMO was obtained by random sequencing. The eukaryotic ex- pression vector was constructed to detect cell apoptosis and senescence activity. Result: The gene of SUMO was cloned with 606 bp and 103 amino acid residues. The expression in HeLa cells could induce the cell apoptosis instead of se- nescence. Conclusion: SUMO from medicinal fungus worth to be investigated for the discovery of new antitumor medicine.

文章引用: 梁一 , 白娟 , 孙慧 (2012) 药用真菌杨树菇SUMO基因及抗肿瘤活性初步鉴定。 医学诊断, 2, 12-15. doi: 10.12677/md.2012.22003

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