Detection of Endosymbionts in Different Bemsia tabaci Populations and Their Effects on Whitefly Host
Abstract: The sweetpotato whitefly Bemisia tabaci (Gennadius) is a globally distributed agricultural pest. The species and infection rates of different endosymbionts in field and laboratory populations of B. tabaci B and Q biotyes were detected by molecular methods. Meawhile, the endosymbionts in B. tabaci Q biotype lab population were inactivated with rifampicin, and then the biology of Wolbachia positive and negative popula-tions of B. tabaci Q biotype were compared. Rusults indicated that all the populations of B. tabaci were in-fected by the primary endosymbiont Candidatus Portiera aleyrodidarum, regardless biotype or population habitat of field or lab, while the infection of secondary endosymbionts Wolbachia and Arsenophonus were marked different in various B. tabaci populations related to biotype and habitat. Inactivated with 1.0mg/ml rifampicin for 48 h, the infection of Wolbachia in B. tabaci Q biotype was completely eliminated, but no ob-vious effect on the Candidatus and Arsenophonu in all the whitefly popualtions. Treated by rifampicin, the fecundity of female adults reduced and the developmental time of F1 offspring extended. Results also showed that the elimination of Wolbachia in B. tabaci led to the increase of male progeny proportion. In general, our current study showed that endosymbionts varied in different B. tabaci populations from various habitats and Wolbachia has significant effects on the biology of its whitefly hosts.
文章引用: 薛夏 , 李绍建 , 陈驹坚 , 任顺祥 , 邱宝利 (2012) 不同生境烟粉虱体内共生菌的检测及其对寄主生物学特性的影响。 农业科学， 2， 5-12. doi: 10.12677/hjas.2012.21002
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