Studies on the Pullulans Polysaccride Produced by Biostat@B Fermentor
Abstract: Pullulans polysaccride, produced from Aureobasidium pullulans, is a kind of extracellular polysaccride. In the current study, in order to change the permeability of cell membrane and solve the problem of linked group of fungi mycelium, the method adding tween-80 and different Ca2+ resources was established. The utilized strain with relatively high polysaccride yield and low pigment level was obtained after the rejuvenation and riddling of long-preserved Aureobasidium pullulans strain. The transformation ratio of polysaccride was increased by 10% - 20% and the pigment content was greatly reduced. By adopting Biostat@B5L fermentor according to the optimum formula, and find the opti-mum aerate speed is 1.5 vvm, and the rate of circle is 500 rpm and culture temperature is 28˚C ± 1.5˚C, and initial pH is 6.5, so a high conversion rate of 65% (35 g/L polysaccride yield)was obtained from the light colour medium. The fer-menting liquor is between milk white and light yellow, and the white primary product can be gained without decolora-tion step.
文章引用: 马金龙 , 姜国斌 , 姚善泾 , 金华 , 王长海 (2012) Biostat@B发酵罐生产低色素短梗霉多糖的研究。 生物过程， 2， 40-44. doi: 10.12677/bp.2012.21007
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