药用真菌杨树菇未知基因1C10克隆及其抗肿瘤活性初步鉴定
The Molecular Cloning and Bioactivity Characterization of Unknown Gene 1C10 from Medicinal Fungus Agrocybe aegerita

作者: 梁一 :广东医学院检验学院临床免疫教研室; 郭莲仙 :广东医学院公共卫生学院劳动卫生与环境卫生教研室; 孙慧 :武汉大学生命科学学院;

关键词: 药用真菌抗肿瘤活性cDNA序列Medicinal Fungus Antitumor Activity cDNA Sequence

摘要:
目的:克隆杨树菇抗肿瘤相关蛋白基因,初步鉴定其活性。方法:构建cDNA文库,随机测序得到杨树菇基因1C10。高通量测序分析其在菌丝体和子实体的差异表达。构建真核表达质粒检测其在HeLa细胞中表达引起的细胞凋亡及衰老的变化,初步鉴定其抗肿瘤活性。结果:得到全长为728 bp的杨树菇基因1C10,编码107个氨基酸,高通量测序分析发现其在子实体中为高表达。在真核细胞中表达会引起肿瘤细胞的凋亡而非衰老。结论:通过构建cDNA文库及测序初步鉴定出具抗肿瘤活性的杨树菇未知基因1C10,为进一步研究其活性及机理奠定基础。
Objective: The antitumor-associated gene of Agrocybe aegerita was cloned to identify the bioactivity. Method: cDNA library was constructed and the gene 1C10 was obtained by random sequencing. The differential ex- pression of the gene was analyzed by high-throughout sequencing. The eukaryotic expression vector was constructed to detect cell apoptosis and senescence activity. Result: The gene of 1C10 was cloned with 728 bp and 107 amino acid residues. The high-throughout sequencing analyse showed that 1C10 was highly expressed in the fruiting body. The expression in HeLa cells could induce the cell apoptosis instead of senescence. Conclusion: 1C10 was identified to have antitumor activity by cDNA library and sequencing, which was fundation for further study.

文章引用: 梁一 , 郭莲仙 , 孙慧 (2012) 药用真菌杨树菇未知基因1C10克隆及其抗肿瘤活性初步鉴定。 医学诊断, 2, 1-5. doi: 10.12677/md.2012.21001

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