ROS Detection in HL60 Based on ALA-PDT
Abstract: With the development of the basic research of photodynamic therapy (PDT) and a wide range of potential clinical applications have be carried out. The monitoring of reactive oxygen species (ROS) which produced in PDT has become a hot issue of PDT research in recent years. In the paper, the status of ROS in HL60 cells were investigated by fluorescence spectra acquired during PDT. The results showed that the largest number of ROS in PDT was achieved at the optimized conditions(the cells incubation time is 4 hours, the added concentration of ALA is 10 mM/L), in which up to 93.2% PDT efficiency for the HL60 Cells can be obtained within 60 minutes.
文章引用: 李莉莉 , 郑泽鳞 , 黄康强 , 熊建文 , 王健 (2011) 基于ALA-PDT的HL60细胞中活性氧的检测。 光电子， 1， 11-15. doi: 10.12677/oe.2011.12003
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