﻿ 基于数字全息显微的牛奶中样本成像方法的研究

# 基于数字全息显微的牛奶中样本成像方法的研究Study on the Imaging Method of Samples Immersed in Milk Based on Digital Holographic Microscopy

Abstract: The imaging of samples immersed in milk media is blurred because of the scattering effect. By utilizing the digital holography technique, the phase and intensity of wave front of the transmitting beam through the non-transparent media can be reconstructed, while the noises introduced by the scattering material can be filtered by digital filtering algorithm. In this paper, the transmission digital holographic microscopic system was applied in imaging of USAF resolution plate immersed in milk. The effect of incident light intensity on imaging is also discussed. The experiment results show that even when the incident light source is too weak to image the sample in milk, by applying digital holographic microscopy, clear reconstructed intensity image of sample in milk can still be obtained. Therefore, digital holography technique is especially suitable for the imaging of samples immersed in milk media.

1. 引言

2. 基于数字全息显微的牛奶中样本成像原理

${I}_{H}\left(x,y\right)={I}_{noise}+{|R|}^{2}+{|O|}^{2}+{R}^{\ast }O+{O}^{\ast }R$ (1)

$u\left(x,y\right)=\Gamma \left(x,y\right)R\left(x,y\right)\left[R*\left(x,y\right)O\left(x,y\right)\right]$ (2)

$Intensity\left(x,y\right)={|u\left(x,y\right)|}^{2}$ (3)

3. 系统与实验

3.1. 透射式数字全息系统

Figure 1. Setup of transmitting digital holographic microscope

3.2. 牛奶中分辨率板成像实验

(a) (b) (c)

Figure 2. USAF resolution board in air medium. (a) digital microscopic hologram; (b) frequency spectrum; (c) intensity

(a) (b) (c)

Figure 3. USAF resolution board in milk medium. (a) digital microscopic hologram; (b) frequency spectrum; (c) intensity

3.3. 入射光强对非透明介质样本成像的影响

(a) (b) (c) (d) (e) (f) (g) (h) (i) (j) (k) (l) (m) (n) (o)

Figure 4. Digital holograms of USAF resolution board in milk medium illuminated by optical sources with multiple intensities. (a)-(o) the intensity of 4.5 mW, 4.2 mW, 3.9 mW, 3.6 mW, 3.3 mW, 3.0 mW, 2.7 mW, 2.4 mW, 2.1 mW, 1.8 mW, 1.5 mW, 1.2 mW, 0.9 mW, 0.6 W and 0.3 mW

(a) (b) (c) (d) (e) (f) (g) (h) (i) (j) (k) (l) (m) (n) (o)

Figure 5. Frequency spectrum of USAF resolution board in milk medium illuminated by optical sources with multiple intensities. (a)-(o) the intensity of 4.5 mW, 4.2 mW, 3.9 mW, 3.6 mW, 3.3 mW, 3.0 mW, 2.7 mW, 2.4 mW, 2.1 mW, 1.8 mW, 1.5 mW, 1.2 mW, 0.9 mW, 0.6 W and 0.3 mW

(a) (b) (c) (d) (e) (f) (g) (h) (i) (j) (k) (l) (m) (n) (o)

Figure 6. Reconstructed intensity of USAF resolution board in milk medium illuminated by optical sources with multiple intensities. (a)-(o) the intensity of 4.5 mW, 4.2 mW, 3.9 mW, 3.6 mW, 3.3 mW, 3.0 mW, 2.7 mW, 2.4 mW, 2.1 mW, 1.8 mW, 1.5 mW, 1.2 mW, 0.9 mW, 0.6 W and 0.3 mW

4. 结论

NOTES

*通讯作者。

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