几种酸性染料共振瑞利散射法测定穿琥宁
Resonance Rayleigh Scattering Method for Determining Patassium Dehydroandrographolide Succinate with Some Acid Dyes

作者: 简易 :川北医学院物理教研室,南充; 陈莲惠 :川北医学院化学教研室,南充;

关键词: 共振瑞利散射法穿琥宁测定染料Resonance Rayleigh Scattering Method Potassium Dehydroandrographolide Succinate Determination Dye

摘要: 在pH 4.5的酸性溶液中,穿琥宁和伊文思蓝、滂胺天蓝、甲基紫等酸性染料结合,导致RRS增强,并产生新的RRS光谱,其最大峰位于720 nm附近。在25 mg?L–1~900 mg?L–1、35 mg?L–1~950 mg?L–1、2 mg?L–1~52 mg?L–1浓度范围内,RRS增强与穿琥宁的浓度成正比。方法具有高灵敏度,其检出限分别为18.5 ng?L–1、32.1 ng?L–1和28.9 ng?mL–1。将该方法用于穿琥宁注射液中穿琥宁含量的测定,结果较满意。

Abstract: In pH 4.5 butter solution, some acid dyes like Evens blue (EB), pontamine blue (PB) and methyl violet (MV) react rapidly with patassium dehydroandrographolide succinate (PDS) to form complexes, which result in great enhancement of resonance Rayleigh scattering(RRS) and a new RRS spectrum. The maximum RRS peak is near 720 nm. RRS intensities are proportional to the concentration of PDS in the range of 25 mg?L–1 - 900 mg?L–1, 35 mg?L–1 - 950 mg?L–1 and 2 mg?L–1 - 52 mg?L–1 separately. The method has high sensitivity and the detect limit for PRS are 18.5 ng?L–1, 32.1 ng?L–1 and 28.9 ng?L–1 separately. The method has been used for the determination of total amounts of PRS in samples with satisfactory results.

文章引用: 简易 , 陈莲惠 (2011) 几种酸性染料共振瑞利散射法测定穿琥宁。 化学工程与技术, 1, 17-21. doi: 10.12677/hjcet.2011.12004

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