Application of the T-RFLP Technique in Microbial Communities Diversity Research

作者: 隋 心 :中国科学院沈阳应用生态研究所;中国科学院研究生院,北京 ; 丁 舒 :长春大学生物科学技术学院; 韩士杰 * , 丁凤英 :中国科学院沈阳应用生态研究所; 黄乃伟 :长白山保护开发区林业局;

关键词: 真菌细菌T-RFLPFungi Bacterial T-RFLP

摘要: 土壤微生物在物质循环转化中具有重要的作用,与森林植被类型、土壤理化性质存在密切的关系。森林土壤微生物多样性及其变化在一定程度上反映了土壤环境的生产力和稳定性,对森林演替,土壤生境改善等有重要意义。末端限制性酶切片段长度多态性分析(T-RFLP)技术具有快捷、高效和可重复性高等优点。本文简要介绍T-RFLP技术的原理和特点,分析这一技术的局限性和优化方法。以及该技术在森林土壤微生物多样性研究中的应用现状展望该技术的发展前景以期能为今后这一领域的研究提供科学依据。

Abstract: Soil microorganism plays an important role in recycling and transformation of material as well as has a close relationship with the type of forest types, soil physical and chemical. The microbial diversity can indicate the station of the forest environment, and play a key function in succession and restoration. Recently, more and more published papers focus on this area. The T-RFLP approach serves as a fast, efficient and reproducible tool. In this review, we introduced the principle and character of T-RFLP technique, analysis of the limitation and optimization method, demonstration the prospects for the development of the technology, in order to provide a scientific basis for future research in this area.

文章引用: 隋 心 , 丁 舒 , 韩士杰 , 黄乃伟 , 丁凤英 (2012) T-RFLP技术在森林土壤微生物多样性研究中的应用。 生物过程, 2, 123-127. doi: 10.12677/BP.2012.23020


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