诺丽内生真菌抗氧化活性的研究及其系统发育树的构建
Studies on Antioxidant Activity of Endo Phytic Fungi Isolated from Morinda citrifolia (Noni) and Construction of Phylogenetic Tree
作者: 马文婷 , 吴友根 * , 胡征波 , 张军锋 , 于靖 ：海南大学热带农林学院，海南 海口;
关键词: 诺丽； 内生真菌； 抗氧化活性； 系统发育树； Morinda citrifolia Linn.； Endophytic Fungi； Antioxidant Activity； Phylogenetic Tree
摘要:Abstract: The secondary metabolites were obtained by using ethyl acetate as solvent for 8 kinds of fermen-tation liquid extraction from endophytic fungi of Noni leaves and fruits. The antioxidant activity was analyzed by DPPH radical scavenging method, ABTS radical scavenging assay and FRAP total reduction capability, and the total polyphenols content (TPC) and total flavonoid content (TFC) of the secondary metabolites were determined by using Folin-reagent method and AlCl3 colorimetric method. The results showed that the antioxidant activities of 8 strains of Noni endophytic fungi were strong, the highest value of DPPH, ABTS and FRAP of the antioxidant capacity was 31.24 mmol/L-Trolox/g DW, 10.92 mmol/L-Trolox/g DW and 9.62 mmol/L, respectively. At 1 mg/mL, the content range of TPC and TFC was 17.99 - 79.03 mg/g, 48.38 - 158.97 mg/g, respectively. The strong antioxidant activity made the Noni endophytic fungi have the important value in exploring the natural antioxidant activity of products.
文章引用: 马文婷 , 吴友根 , 胡征波 , 张军锋 , 于靖 (2017) 诺丽内生真菌抗氧化活性的研究及其系统发育树的构建。 植物学研究， 6， 429-438. doi: 10.12677/BR.2017.66056
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