Simultaneous Determination of Protopine and Palmatine Chloride in Qingnaozhitong Capsule by HPLC
Abstract: Qingnaozhitong Capsule is mainly composed with Radix Paeoniae Alba, Ligusticum wallichii, Radices Paeoniae Alba, Rhizoma Corydalis, Tribulus terrestris, Angelica dahurica, Acanthopanax Senticosus and Corydalis Amabilis which are processed by steam distillation, water extraction and alcohol extraction. They have the effect of activating blood, relieving pain and calming the nerves. The content of paeoniflorin in Radix Paeoniae Alba was determined by the existing quality standards. Radices Paeoniae Alba, Corydalis Amabilis, Rhizoma Corydalis and Acanthopanax Senticosus were identified by TLC. Paeoniflorin is from water extraction, in order to control the quality of products further. HPLC was used to determinate protopine hydrochloride and Pakistan Martin, which is extracted by ethanol from Rhizoma Corydalis. The results show that the method is sensitive, rapid and reliable, and can be used for quality control of Qingnaozhitong capsule.
文章引用: 胡正明 , 张 焱 , 嵇 晶 , 程建明 (2017) HPLC法同时测定清脑止痛胶囊中原阿片碱和盐酸巴马汀含量。 药物资讯， 6， 127-132. doi: 10.12677/PI.2017.65021
 国家药典委员会. 中华人民共和国药典[S]. 一部. 北京中国医药出版社, 2015: 279.
 National Pharmacopoeia Committee. (2015) The Pharmacopoeia of the People’s Republic of China. Beijing Chinese Medicine Press, Beijing, 279.
 袁劲松, 古桂花, 曾薇, 等. HPLC法同时测定夏天无中11种生物碱的含量[J]. 中药材, 2013, 36(8): 1283-1287.
 Yuan, J.-S., Gu, G.-H., Ceng, W., et al. (2013) HPLC Method to Determine the Content of 11 Kinds of Alkaloids in Corydalis. Journal of Traditional Chinese Medicine, 36, 1283-1287.
 龙项, 胡还甫. 高效液相色谱法测定不同产地夏天无药材中5种生物碱的含量[J]. 中国医院药学杂志, 2013, 33(13): 1104-1106.
 Xiang, L. and Hu, H.-F. (2013) High Performance Liquid Chromatography Determination of Different Origin No Medicinal Material Content of 5 Kinds of Alkaloids in Summer. Chinese Journal of Hospital Pharmacy, 33, 1104-1106.
 曾岗, 张洁. 高效液相色谱法测定夏天无胶囊中原阿片碱、盐酸巴马汀和比枯枯灵碱的含量[J]. 中南药学, 2014(6): 588-591.
 Zeng, G. and Zhang, J. (2014) The High Performance Liquid Chromatography (HPLC) Method Was Used to Determine the Content of Opioids in the Summer Capsules without Capsules, Bamadine Hydrochloride, and the Ratio of Paraquinine. Chinese Medicine, No. 6, 588-591.
 邵军. 夏天无提取物质量标准研究[D]: [硕士学位论文]. 南昌: 南昌大学医学院, 2015.
 Jun, S. (2015) Research on the Quality of Free Extract in Summer. Nanchang University Medical School, Nanchang.