神经干细胞移植对缺血缺氧性脑损伤大鼠的动作电位的影响
The Effect of Transplantation of Neural Stem Cells on the CMAP in Rats with Hypoxic-Ischemic Brain Injury

作者: 徐奕鹏 , 侯 甜 :新疆维吾尔自治区人民医院康复科,新疆 乌鲁木齐;

关键词: 神经干细胞移植动作电位缺血缺氧性脑损伤Neural Stem Cells Transplantation CMAP Hypoxic-Ischemic Brain Injury

摘要:
目的:探讨NSCs移植对治疗新生儿缺血缺氧性脑病的的可行性。方法:采用E14胎鼠大脑皮层制备NSCs。将40只Wistar大鼠随机分配到NSCs组(20只)、HIE组(20只),对7日龄NSCs移植组新生大鼠在建模后72 h进行经脑室NSCs移植,于移植后3周、7周进行运动诱发电位测定。结果:术后3周、7周时NSCs移植组实验鼠后肢股四头肌复合动作电位(CMAP)与对照组相比引出波幅明显偏高(P < 0.01)。结论:NSCs移植可改善缺血缺氧性损伤大鼠的运动功能。经脑室NSCs移植对于缺血缺氧性脑病的新生儿具有很大的治疗潜力,为今后成功防治小儿脑瘫脑提供了新的可行性途径。

Abstract: Objective: To explore the feasibility of NSCs transplantation for the treatment of hypoxic-ischemic encephalopathy in infants. Methods: A total of 40 two-day-old neonatal Wistar rats were randomly divided into group A and group B (20 rats each group). A total of 40 two-day-old neonatal Wistar rats were randomly divided into group NSCs and group HIE (20 rats each group). Intraventricular transplantation of exdogenous NSCs was operated 72 hrs after surgery. Motor Evoked Potential and Pathology Examination were evaluated at 3 weeks and 7 weeks after operation. Results: At 3rd and 7th week after operation, the NSCs group rats’s hindlimb quadriceps CMAP draws out the wave amplitude to be higher compared with control group (P < 0.01). Conclusions: Intraventricular transplantation of exdogenous NSCs can apparently improve behavioral function. It is suggested that intraventricular transplantation of NSCs is of a great potential feasibility for the treat- ment of hypoxic-ischemic encephalopathy in infants.

文章引用: 徐奕鹏 , 侯 甜 (2017) 神经干细胞移植对缺血缺氧性脑损伤大鼠的动作电位的影响。 临床医学进展, 7, 230-234. doi: 10.12677/ACM.2017.74038

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