两种爱德华氏菌MALDI-TOF-MS检测方法的建立与应用
Establishment and Application of MALDI-TOF-MS Methods for Two Types of Edwardsiella
作者: 吴 斌 , 刘卉秋 , 赵亮 , 臧玉婷 , 关 鹏 ：辽宁出入境检验检疫局，辽宁 大连;
关键词: MALDI-TOF-MS； 鮰爱德华氏菌； 迟钝爱德华氏菌； 检测； 鉴定； MALDI-TOF-MS； Edwardsiella ictaluri； Edwardsiella tarda； Detection； Identification
摘要:Abstract: In this paper, the rapid MALDI-TOF-MS method was established to detect and identify Edwardsiella ictaluri and Edwardsiella tarda. The stability and repeatability of MALDI-TOF-MS were verified by Vibrio parahaemolyticus, Edwardsiella ictaluri, Edwardsiella tarda, Aeromonas hydrophila, Aeromonas salmonicida. The strains from different sources were detected in order to confirm the accuracy of this method. Through the comparison of MALDI-TOF-MS technology and traditional identification methods, the results showed that MALDI-TOF-MS were better than traditional methods in detection time, repeatability, stability, and other aspects. MALDI-TOF-MS could be used for Edwardsiella ictaluri and Edwardsiella tarda in daily detection.
文章引用: 吴 斌 , 刘卉秋 , 赵亮 , 臧玉婷 , 关 鹏 (2017) 两种爱德华氏菌MALDI-TOF-MS检测方法的建立与应用。 水产研究， 4， 119-123. doi: 10.12677/OJFR.2017.43019
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