Comparison of Different Electrodes for F-VEP Recording in Rats
Abstract: Objective: To explore a better electrode setting method for F-VEP detection in rats, the difference of different F-VEP recording methods was compared. Methods: 18 SD-rats were randomly divided into three groups. The skull implanted electrode, the traditional hypodermic needle electrode and the modified hypodermic needle electrode were respectively recorded in each group. The recording results are the latency of N1 wave and P1 wave and the amplitude of N1-P1 wave, and were statistically analyzed by SPSS software. Results: The latency of P1 wave in the skull implanted electrode group was significantly advanced than the traditional subcutaneous needle electrode group (P = 0.002); the latency of P1 wave in modified subcutaneous needle electrode group was significantly advanced than the traditional subcutaneous needle electrode group (P = 0.025); and no significantly difference was found between the skull implanted electrode group and the modified subcutaneous needle electrode (P > 0.05). The amplitude of the N1-P1 wave in the skull implanted electrode group were significantly larger than two subcutaneous needle electrode groups (P = 0.028/P = 0.011); and no difference was showed between the two subcutaneous needle electrode group in amplitude of the N1-P1 wave (P > 0.05). Only the skull implanted electrode group had adverse events, while subcutaneous needle electrode groups were all well. Conclusion: The skull implanted electrode recording method has excellent sensitivity and repeatability, and the traditional subcutaneous needle electrode recording method is well in animal’s safety, but the modified subcutaneous needle electrode recording method retains the security advantage and simultaneously enhances the sensitivity and repeatability. So the modified subcutaneous needle electrode recording method is effective in F-VEP recording in rats.
文章引用: 张榆欣 , 鲍义琴 , 徐 颖 , 杨 晖 (2017) 大鼠视觉诱发电位记录方法比较研究。 眼科学， 6， 101-106. doi: 10.12677/HJO.2017.63017
Huang, T., Wen, Y., Chang, C., et al. (2017) Early Methylprednisolone Treatment Can Stabilize the Blood-Optic Nerve Barrier in a Rat Model of Anterior Ischemic Optic Neuropathy (rAION). Investigative Ophthalmology & Visual Science, 58, 1628-1636.
Aranda, M.L., Dorfman, D., Sande, P.H., et al. (2015) Experimental Optic Neuritis Induced by the Microinjection of Lipopolysaccharide into the Optic Nerve. Experimental Neurology, 266, 30-41.
Yu, M., Sturgill-Short, G., Ganapathy, P., et al. (2012) Age-Related Changes in Visual Function in Cystathionine-Beta-Synthase Mutant Mice, a Model of Hyperhomocyste-inemia. Experimental Eye Research, 96, 124-131.
You, Y., Klistorner, A., Thie, J., et al. (2011) Improving Repro-ducibility of VEP Recording in Rats: Electrodes, Stimulus Source and Peak Analysis. Documenta Ophthalmologica, 123 109-119.
You, Y., Gupta, V.K., Chitranshi, N., et al. (2015) Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination. Journal of Visualized Experiments, 101, e52934.
You, Y., Klistorner, A., Thie, J., et al. (2011) Latency Delay of Visual Evoked Potential Is a Real Measurement of Demyelination in a Rat Model of Optic Neuritis. Investigative Ophthalmology & Visual Science, 52, 6911-6918.
 郭群, 谢蓓, 顾永昊, 等. 植入电极法记录大鼠视觉诱发电位[J]. 国际眼科杂志, 2007(6): 1530-1534.