Sperm Chromatin Diffusion Test and Detection of Sperm DNA Integrity by Acridine Orange Staining
Abstract: Objective: To analyze and compare the accuracy and utility of determining sperm DNA integrity by sperm chromatin dispersion (SCD) test and acridine orange staining test (AOT). Methods: The level of DNA fragmentation was determined by SCD test and AOT in 32 adult healthy fertile men (control group) and 27 idiopathic oligozoospermia (IO) patients. Sperm nuclei with large DNA dispersion halos or with medium-sized halos were normal and nuclei with small-sized halos or no halo were abnormal. Sperm DNA strand breaks were tested by AOT of green for native DNA and red for denatured DNA. Results: The mean percentage of sperm nuclei with large halos, medium-sized halos, small-sized halos and no halo in patients with IO was (51.8 ± 17.1)%, (9.4 ± 8.6)%, (11.9 ± 5.8)% and (26.7 ± 10.6)%, but in control group it was (73.2 ± 6.2)%, (14.8 ± 5.7)%, (6.8 ± 2.9)% and (5.3 ± 2.2)%, respectively, there was a significantly difference in the IO and control group (t = 4.256, P < 0.01; t = 5.1849, P < 0.05; t = 3.6967, P < 0.01; t = 5.7971, P < 0.01). IO patients showed significantly higher DNA fragmentation (38.1% ± 9.5%) in their spermatozoa compared to healthy males (12.1% ± 5.2%, P < 0.01) determined by SCD test. No significant differences in the AOT outcome were seen between healthy males and IO patients (P > 0.05). Conclusion: The SCD is an effective test in sperm DNA fragmentation as a screening procedure to determine semen quality during basic infertility investigation for clinical use. The presence of sperm DNA fragmentation may lead to male infertility.
文章引用: 房振亚 , 张美华 , 李安娜 , 邱毅 (2017) 精子染色质扩散实验及吖啶橙染色检测精子DNA完整性研究。 医学诊断， 7， 43-49. doi: 10.12677/MD.2017.73008
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