Nuclear Factor Erythroid 2-Related Factor 2 Deficiency (Nrf2-/-) Enhances the Cigarette Smoke Extract-Induced Immuno-Stimulus Response of in Mice—Nrf2 Deficiency Affects the Immuno-Stimulus Response of Mice
Abstract: Objective: To investigate the variation of spleen lymphocyte subsets percentages and abdominal macrophage inflammatory factor of the cigarette smoke extract-induced Nrf2-/- mice, and to dis-cuss the role of Nrf2-/- in cigarette smoke extract-induced immuno-stimulus response of mice. Methods: 36 female C57BL/6 WT and Nrf2-/- mice were randomly divided into 2 groups, respectively. Two groups of WT and Nrf2-/- mice drank pure water containing DMSO dissolved cigarette smoke extract for 4 months continuously; the other two groups’ mice drank pure water containing the same amount of DMSO for four months. Spleens were harvested for taking pictures and necrosis scores; the single cell suspension of spleen tissue was prepared for splenocyte counting and the variation of spleen lymphocyte subsets was analyzed with flow cytometry. Meanwhile, the abdominal macrophages were harvested for the detecting of the related inflammatory factor. Results: Nrf2-/- prompted the spleen swelling with necrosis and lowered the number of splenocytes of cigarette smoke extract-induced mice; Nrf2-/- increased the percents of immune regulation related spleen total T cells and CD8 T cells and reduced the B cells; Nrf2-/- reduced the CD4 and CD8 TCM or TEM cells and increased the TE cells; Nrf2-/- enhanced the majority of inflammatory cytokines, colony stimulating factors and chemokines expression in abdominal macrophage of cigarette smoke extract-induced mice. Conclusion: Nrf2-/- enhances the cigarette smoke extract-induced natural immune and regulating immune response in mice.
文章引用: 徐小解 , 丁燕超 , 黄朝峰 (2017) Nrf2基因缺陷增强烟雾浓缩物对小鼠的免疫刺激反应—Nrf2基因缺陷影响着小鼠的免疫应激反应。 免疫学研究， 5， 1-9. doi: 10.12677/IS.2017.51001
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