不同生长时期碱蓬在Pb2+、Cd2+单一及复合胁迫下的生理生化响应
Physiological and Biochemical Responses of Suaeda salsa to Pb2+,Cd2+ in Single and Combined Stress at Different Growth Stages

作者: 于晓磊 , 赵欣园 , 杨 佳 :浙江万里学院生物与环境学院,浙江 宁波; 王路遥 , 王趁义 * , 滕丽华 , 胡 杰 :浙江万里学院生物与环境学院,浙江 宁波 ;

关键词: 碱蓬Pb2+Cd2+胁迫生理生化i>Suaeda salsa Lead Cadmium Stress Physiology and Biochemistry

摘要:
由于人类活动及工业化的迅速发展,沿海滩涂湿地正在遭受前所未有的污染和破坏,其中铅、镉污染是主要的污染之一,而碱蓬有较强的重金属耐受性和耐盐性,在修复污染的同时也具有较高的经济价值。为研究不同生长时期碱蓬在Pb2+、Cd2+胁迫下的生理生化响应,采用蛭石室外培养,对碱蓬进行Pb2+、Cd2+单一及复合污染处理,结果表明:Pb2+、Cd2+单一及复合胁迫下,碱蓬幼苗期与花期的株高、超氧化物歧化酶(SOD)活性、脯氨酸(Pro)含量、丙二醛(MDA)含量均呈显著差异(P<0.05)。碱蓬在抵御外界Pb2+、Cd2+胁迫时,以SOD活性的快速响应为先,Pro的渗透调节抵御为后,MDA的无序变化实时表征植物的受损状况。总之,碱蓬对Pb2+、Cd2+的胁迫有较强的自我调节能力,具有修复Pb2+、Cd2+污染的潜力,这为进一步利用盐生植物碱蓬修复滨海滩涂等高盐碱土壤,以及抵御Pb2+、Cd2+等重金属复合污染的损伤机制研究提供了理论依据。

Abstract: Due to the rapid development of human activities and industrialization, tidal wetlands are suffering from unprecedented pollution and destruction b which lead and cadmium pollution is one of the heavy metal pollution. While Suaeda salsa has strong heavy metal tolerance, in the removal of pollution at the same time also has high economic value. In order to study the growth and physiological and biochemical responses of Suaeda salsa under different Pb2+ and Cd2+ concentration, the plant was cultured by vermiculite outdoors, single and combined pollution of Pb2+ and Cd2+ was carried out. The results showed that the plant height and contents of SOD, Pro and MDA in seedling stage and flowering stage of Suaeda salsa were significantly different. When the Suaeda salsas were responded to the Pb2+, Cd2+ single and combined stress, SOD activities responded quickly at first, Pro’s osmotic adjustment would be activated after SOD, the disorderly MDA contents real-time indicated the damaged conditions of cell membrane. In conclusion, Suaeda salsa had strong self-adjustable mechanism when under the Pb2+, Cd2+ stress treatment and which have potential for restore the Pb2+, Cd2+ pollution, which provide a theoretical basis for injury mechanism in which halophytes Suaeda salsa restore high saline areas, such as coastal wetlands and resist the heavy metals contamination.

文章引用: 于晓磊 , 王路遥 , 赵欣园 , 王趁义 , 滕丽华 , 杨 佳 , 胡 杰 (2017) 不同生长时期碱蓬在Pb2+、Cd2+单一及复合胁迫下的生理生化响应。 农业科学, 7, 120-127. doi: 10.12677/HJAS.2017.72015

参考文献

[1] 张韵, 蒲新明, 黄丽丽, 等. 我国滨海湿地现状及修复进展[C]//中国环境科学学会. 2013中国环境科学学会学术年会论文集(第六卷), 2013: 5743-4746.

[2] 刘俊祥, 孙振元, 巨关升, 等. 结缕草对重金属镉的生理响应[J]. 生态学报, 2011, 31(20): 6149-6156.

[3] 贺靖舒, 丁继军, 潘远智, 等. 石竹(Dianthus chinensis)对土壤镉胁迫的生理生化响应[J]. 四川农业大学学报, 2013, 31(3): 290-295.

[4] Finger-Teixeira, A., Ferrarese, M.D.L.L., Soares, A.R., et al. (2010) Cadmium-Induced Lignification reStricts Soybean Root Growth. Ecotoxicology & Environmental Safety, 73, 1959-1964.
https://doi.org/10.1016/j.ecoenv.2010.08.021

[5] Gichner, T., Žnidar, I., Száková, J. (2008) Evaluation of DNA Damage and Mutagenicity Induced by Lead in Tobacco Plants. Mutation Research/Genetic Toxicology & Environmental Mutagenesis, 652, 186-190.
https://doi.org/10.1016/j.mrgentox.2008.02.009

[6] Sharma, P., Dubey, R.S. (2005) Lead Toxicity in Plant. Brazilian Journal of Plant Physiology, 17, 35-52.
https://doi.org/10.1590/S1677-04202005000100004

[7] Reddy, A.M., Kumar, S.G., Jyothsnakumari, G., et al. (2005) Lead Induced Changes in Antioxidant Metabolism of Horsegram (Macrotyloma uniflorum, (Lam.) Verdc.) and Bengalgram (Cicer arietinum, L.). Chemosphere, 60, 97-104.
https://doi.org/10.1016/j.chemosphere.2004.11.092

[8] Chen, H.L., Lai, H.Y., Wang, S.M., et al. (2010) Effect of Biosolids and Cd/Pb Interaction on the Growth and Cd Accumulation of Brassica rapa, Grown in Cd-Contaminated Soils. Water Air & Soil Pollution, 206, 385-394.
https://doi.org/10.1007/s11270-009-0114-9

[9] 熊春晖,许晓光,卢永恩, 等. 铅镉复合胁迫下莲藕对铅镉的富集及其生理变化[J]. 园艺学报, 2012, 39(12): 2385- 2394.

[10] 刘大林, 杨俊俏, 刘兆明, 等. 镉、铅胁迫对草地早熟禾幼苗生理的影响[J]. 草业科学, 2015, 32(2): 224-230.

[11] 梁烜赫, 曹铁华. 重金属对玉米生长发育及产量的影响[J]. 玉米科学, 2010, 18(4): 86-88.

[12] 孙永林. 铅对黄瓜叶片丙二醛、脯氨酸和可溶性总糖含量的影响[J]. 长江蔬菜(学术版), 2008, 1(24): 28-30.

[13] 张爱琴, 庞秋颖, 阎秀峰. 碱蓬属植物耐盐机理研究进展[J]. 生态学报, 2013, 33(12): 3575-3583.

[14] 李超峰, 葛宝明, 姜森颢, 等. 碱蓬对盐碱及污染土壤生物修复的研究进展[J]. 土壤通报, 2014, 45(4): 1014- 1019.

[15] 高世珍, 赵兴茹, 崔世茂, 等. 典型持久性有机污染物在翅碱蓬中的分布特征[J]. 环境科学, 2010, 31(10): 2456- 2461.

[16] 郑春芳, 陈琛, 彭益全, 等. 海水养殖废水灌溉对碱蓬和海蓬子生长和品质的影响[J]. 浙江农业学报, 2012, 24(4): 663-669.

[17] 李学刚, 袁华茂, 许思思, 等. 胶州湾滨海湿地盐渍土壤中重金属的聚集与分散特性研究[J]. 海洋科学, 2011, 35(7): 88-95.

[18] 李杏, 项学敏, 周集体, 等. 盐生植物碱蓬在土壤修复及废水处理中的研究现状[J].江苏环境科技, 2007, 20(1): 53-54.

[19] 张海波, 崔继哲, 曹甜甜, 等. 大豆出苗期和苗期对盐胁迫的响应及耐盐指标评价[J]. 生态学报, 2011, 31(10): 2805-2812.

[20] Min, Q., Qin, L.N., Liu, Y.J., et al. (2014) The Comparison of Two Methods of Testing Superoxide Dismutase Activity. Journal of Food Safety & Quality, 1, 3318-3323.

[21] Kusvuran, A. (2015) The Effects of Salt Stress on the Germination and Antioxidative Enzyme Activity of Hungarian vetch (Vicia pannonica Crantz.) Varieties. Legume Research, 38, 51-59.
https://doi.org/10.5958/0976-0571.2015.00009.0

[22] Rady, M.M., Mohamed, G.F., Abdalla, A.M., et al. (2015) Integrated Application of Salicylic Acid and Moringa oleifera Leaf Extract Alleviates the Salt-Induced Adverse Effects in Common Bean Plants/ International Journal of Agricultural Technology, 11, 1595-1614.

[23] 周玉卿, 赵九洲, 韩玉林. 铅、镉及其复合胁迫对黄菖蒲幼苗生长和生理抗性的影响[J]. 湿地科学, 2012, 10(4): 487-491.

[24] 温瑀, 穆立蔷. 土壤铅、镉胁迫对4种绿化植物生长、生理及积累特性的影响[J]. 水土保持学报, 2013, 27(5): 234-239.

[25] 徐学华, 黄大庄, 王连芳, 等. 土壤铅、镉胁迫对红瑞木生长及生理生化特性的影响[J]. 水土保持学报, 2009, 23(1): 213-221.

[26] 陆海燕, 刘志辉, 吕光辉. 镉污染下芦苇叶片丙二醛、脯氨酸及SOD保护酶反应研究[J]. 干旱区资源与环境, 2013, 27(8): 171-175.

[27] 郭天荣, 陈丽萍, 冯其芳, 等. 铝、镉胁迫对空心菜生长及抗氧化特性的影响[J]. 核农学报, 2015, 29(3): 571-576.

[28] 何洁, 陈旭, 王晓庆, 等. 翅碱蓬对滩涂湿地沉积物中重金属Cu, Pb的累积吸收[J]. 大连海洋大学学报, 2012, 27(6): 539-545.

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