Correlation between Galectin-3 Protein and Foxp3+, IL-17+ Lymphocytes in Colon Cancer Microenvironment
Abstract: Background: Galectin-3 (gal-3) involves in tumor development, progression, and regulates im-mune cells activity. Th17 cells and T regulatory cells (Treg) in tumor environment could affect tumor progression and prognosis. Recently few researches investigated the relation between gal-3 and immune cells in colon cancer microenvironment, therefore this research aimed to demonstrate the correlation between gal-3 and Foxp3+, IL-17A+ lymphocytes in colon cancer microenvironment, for further investigating the effect on immune microenvironment from colon cancer- associated protein. Methods: From 2011 to 2012, fifty colon cancer tissue samples and 10 adjacent normal tissues after resection in Affiliated Cancer Hospital of Guangzhou Medical University were collected. Expressions of gal-3, IL-17A and Foxp3 were detected by using immuno histochemistry (IHC) technique. Correlation and significance among these three markers were analyzed with clinical-pathological data. Results: Gal-3 was mainly expressed in cytoplasm of tumor cells, Foxp3 was often found in cellular nuclear of stroma lymphocytes, and IL-17A was expressed in cytoplasm of stroma lymphocytes. Analysis showed the positive expressions of gal-3, Foxp3 and IL-17A were all significantly higher than in normal colon tissues. Expressions of gal-3 in late stage cases were much higher than in early cases, as well as the Foxp3+ lymphocytes and IL-17A+ lymphocytes. Univariate analysis revealed stroma infiltrating Foxp3+ lymphocytes and IL-17A+ lymphocytes had positive correlation with tumoral gal-3 expression (r = 0.608, P < 0.001, r = 0.289, P = 0.042), which means the higher expression of gal-3 in tumor was, the more stroma infiltrating Foxp3+ lymphocytes and IL-17A+ lymphocytes were. Conclusion: Gal-3 might promote Foxp3+ lymphocytes and IL-17A+ lymphocytes infiltration in tumor microenvironment and involve in colon cancer growth and development.
文章引用: 李昌宾 , 黄志良 , 李楠 , 刘海鹰 (2017) 结肠癌galectin-3蛋白的表达与微环境Foxp3+、IL-17+淋巴细胞的相关性。 世界肿瘤研究， 7， 32-39. doi: 10.12677/WJCR.2017.72006
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