不同配比组方的百部–紫菀药对咳嗽变异性哮喘大鼠Th1/Th2及Th17/Treg细胞因子平衡影响
Study on the Effects of Couplet Medicine Baibu-Ziwan with Different Proportions on Cytokines Level of Type Th1/Th2 and Th17/Treg in Cough Variant Asthma Rats

作者: 姜登钊 * , 刘可越 :九江学院药学与生命科学学院,江西省系统生物医学重点实验室,江西 九江; 李辉敏 :九江学院基础医学院,江西 九江;

关键词: 药对百部–紫菀变异性哮喘配伍细胞因子Couplet Medicine Baibu-Ziwan Cough Variant Asthma Compatibility Cytokines

摘要:
目的:探讨药对百部–紫菀平喘作用机制并探索配伍比例对药对的药效作用影响。方法:建立SD大鼠咳嗽变异性哮喘(CVA)模型,灌胃百部–紫菀不同配伍比例提取物,采用Elisa方法检测支气管肺泡灌洗液(BALF)中IL-4,INF-γ,IL-17及IL-10含量,并测定BALF中的炎性细胞变化,比较组间差异。结果:药对百部-紫菀给药后,CVA大鼠Thl/Th2及Th17/Treg水平失衡均得到部分纠正,同时嗜酸性粒细胞、淋巴细胞、中性粒细胞等炎性细胞水平明显降低,统计学显示具有显著性差异。配伍比例对药效的作用具有一定程度的影响,配伍1:1时的药效作用最强。结论:药对百部–紫菀对CVA大鼠的平喘机制与纠正Thl/Th2与Th17/Treg细胞平衡,减轻炎性细胞浸润有关,配伍比例对药对的药效作用具有一定程度的影响。

Abstract: Objective: To study the antiasthma mechanism of couplet medicine Baibu-Ziwan and investigate the pharmacodynamic effects on the proportion compatibility. Method: Cough variant asthma (CVA) rats were established and orally administrated by the extractions of Baibu-Ziwan with dif-ferent compatibility. The IL-4, INF-γ, IL-17 and IL-10 levels in bronchoalveolar lavage fluid (BALF) were determined by Elisa method and the numbers of inflammatory cells in BALF changes in lung were measured, respectively. The differences were tested among the groups by SPSS 19.0 software. Results: The levels of Thl/Th2 and Th17/Treg were both corrected after administration of the extractions of Baibu-Ziwan with inflammatory cells (eosinophil, lymphocytes and neutrophil) decreased in CVA group (p < 0.05). Conclusion: The antiasthma mechanism of couplet medicine Baibu-Ziwan on CVA rats may be related to the regulating imbalance of Thl/Th2 and Th17/Treg and the decreased infiltration of inflammatory cells, and the proportion compatibility had some influences on its pharmacodynamic effects.

文章引用: 姜登钊 , 李辉敏 , 刘可越 (2017) 不同配比组方的百部–紫菀药对咳嗽变异性哮喘大鼠Th1/Th2及Th17/Treg细胞因子平衡影响。 药物资讯, 6, 6-11. doi: 10.12677/PI.2017.61002

参考文献

[1] 许东风. 变异性哮喘临床分析[J]. 现代预防医学, 2007, 34(1): 199.

[2] Niimi, A. (2008) Cough Variant Asthma: A Major Cause of Chronic Cough. Clinical Pulmonary Medicine, 15, 189- 196.
https://doi.org/10.1097/CPM.0b013e31817e3059

[3] Niimi, A., Ohbayashi, H., Sagara, H., et al. (2013) Cough Variant and Cough-Predominant Asthma Are Major Causes of Persistent Cough: A Multicenter Study in Japan. Journal of Asthma, 50, 932-937.
https://doi.org/10.3109/02770903.2013.823444

[4] 赵一菊, 肖正伦. 咳嗽变异性哮喘的研究进展[J]. 医学综述, 2012, 18(13): 2067-2069.

[5] 胡军旗, 郎雅丽, 张副兴, 等. 止嗽散治疗咳嗽变异性哮喘疗效评价[J]. 中医学报, 2015, 30(3): 333-336.

[6] 冼峰. 止嗽散加味治疗咳嗽变异性哮喘43例临床观察[J]. 中医药导报, 2014, 20(3): 95-96.

[7] Shi, Y.H., Shi, G.C., Wan, H.Y., et al. (2011) Coexistence of Thl/Th2 and Thl7/Treg Imbalances in Patients with Allergic Asthma. Chinese Medical Journal, 124, 1951-1956.

[8] 梁建卫, 张玉振, 梁敏. 疏肝化瘀养阴清肺方对CVA大鼠IL-6、IL-8的实验研究[J]. 生物医学工程研究, 2009, 28(2): 148-150.

[9] 徐姗, 苏梦琪, 吕伟红, 等. 异丁斯特对支气管哮喘豚鼠Th2型细胞因子白介素4、白介素13 及嗜酸粒细胞趋化因子的影响[J]. 中华哮喘杂志, 2009, 3(5): 346-350.

[10] 胡鑫, 沈唯薇. 难治性哮喘中Th1/Th2失衡机制[J]. 华西医学, 2011, 26(9): 1428-1431.

[11] Dechene, L. (2002) Thl/Th2 Immune Response. Journal of Allergy and Clinical Immunology, 110, 539-540.
https://doi.org/10.1067/mai.2002.127436

[12] Estelle, B., Yijun, C., Wenda, G., et al. (2006) Reciprocal Developmental Pathways for the Generation of Pathogenic Effector TH17 and Regulatory T Cells. Nature, 441, 235-238.
https://doi.org/10.1038/nature04753

[13] 黄东晖, 吴少丽, 王慧贤, 等. 喘可治注射液通过调节Th17/Treg平衡抑制哮喘模型小鼠气道炎症的实验研究[J]. 中药材, 2014, 37(3): 478-481.

[14] Choi, I.S., Cui, Y., Koh, Y.A., et al. (2008) Effects of Dehydroepiandrosterone on Th2 Cytokine Production Inperipheral Blood Mononuclear Cells from Asthmatics. The Korean Journal of Internal Medicine, 23, 176-181.
https://doi.org/10.3904/kjim.2008.23.4.176

[15] Aggarwal, S. and Gurney, A.L. (2002) IL-17: Prototype Member of an Emerging Cytokine Family. Journal of Leukocyte Biology, 71, 1-8.

[16] 上官文姬. 支气管哮喘免疫学发病机制的研究进展[J]. 上海交通大学学报(医学版), 2009, 29(3): 602-606.

[17] 谭晓梅, 郭阳, 余林中, 等. 麻黄-杏仁药对配伍及不同配比对急性毒性及平喘作用的影响[J]. 中药药理与临床, 2013, 29(1): 82-84.

[18] 襄萍, 徐朝晖, 战光绪, 等. 药对麻黄地龙配比及平喘作用机制的研究[J]. 中国中药杂志, 2006, 31(3): 236-239.

分享
Top