中药短柱肖菝葜根部位化学成分的研究
Chemical Constituents from the Root of Heterosmilax yunnanensis Gagnep

作者: 杨爱岗 :第二军医大学药学院,上海 ;北京振东光明药物研究院有限公司,北京; 叶 霁 , 梅丽娟 :第二军医大学药学院,上海; 海丽娜 , 秦文杰 , 李明花 :北京振东光明药物研究院有限公司,北京; 张卫东 :第二军医大学药学院,上海 ;

关键词: 白土苓短柱肖菝葜大泽明素苏铁素复方苦参注射液Rhizoma heterosmilacis Heterosmilax yunnanensis Gagnep. Macrozamin Cycasin Compound Kushen Injection

摘要:
短柱肖菝葜根部入药,习称“白土苓”,是抗肿瘤药物复方苦参注射液的味药之一,对其的化学成分和药理活性研究较少。本文主要对中药短柱肖菝葜根部位中的高极性化学成分展开研究,从中共分离和鉴定了12个化合物:大泽明素(1)、苏铁素(2)、3,4,5-三羟基苯甲醛(3)、3,4-二羟基苯甲醛(4)、4-羟基苯甲酸(5)、3,4-二羟基苯甲酸(6)、2,5-二羟基苯甲酸(7)、3,5-二甲氧基-4-羟基苯甲酸(8)、反式桂皮酸(9)、4-乙氧基-苯丙烯酸(10)、4-(二甲氧基甲基)-苯酚(11)和N-3-(3,5-dihydroxy-4,4-dimethyl-1-oxopentyl)- β-Alanine (12)。除化合物1以外,其他化学成分均是首次从该属植物中分离得到,该项研究丰富了短柱肖菝葜的物质基础。

Abstract: The root of Heterosmilax yunnanensis Gagnep. is the main component of anti-cancer drug Com-pound Kushen Injection. The chemical constituents in its high polarity fraction were investigated in this research. Twelve compounds were isolated from 30% ethanol macroporous resin fractions of the root of Heterosmilax yunnanensis Gagnep. and identified as: macrozamin (1), cycasin (2), 3,4,5-trihydroxy benzaldehyde (3), 3,4-dihydroxy benzaldehyde (4), 4-hydroxy benzoic acid (5), 3,4-dihydroxy benzoic acid (6), 2,5-dihydroxy-benzoic acid (7), 3,5-dimethoxyl -4-hydroxy benzoic acid (8), trans-Cinnamic acid (9), 4-ethyoxyl-Cinnamic acid (10), 4-(dimeth-oxymethyl)-phenol (11), N-3-(3,5-dihydroxy-4,4-dimethyl-1-oxopentyl)-β-Alanine (12) respectively. All compounds except 1 are firstly isolated from the Heterosmilax yunnanensis Gagnep. These compounds can support abundant material basis for the further research of Heterosmilax yunnanensis Gagnep.

文章引用: 杨爱岗 , 叶 霁 , 梅丽娟 , 海丽娜 , 秦文杰 , 李明花 , 张卫东 (2016) 中药短柱肖菝葜根部位化学成分的研究。 中医学, 5, 125-131. doi: 10.12677/TCM.2016.54020

参考文献

[1] 赖云峰, 陆金健, 王一涛, 等. 基于市场状况的抗肿瘤中药注射剂分析[J]. 世界科学技术—中医药现代化, 2012, 14(5): 1958-1962.

[2] 张文谨, 海丽娜, 连增林. 复方苦参注射液抗肿瘤作用及其机制实验研究进展[J]. 中国中医药信息杂志, 2012, 19(8): 101-103.

[3] Zhao, Z.Z., Fan, H.T., Lin., H.S., et al. (2014) Fufang Kushen Injection Inhibits Sarcoma Growth and Tumor-Induced Hyperalgesia via TRPV1 Signaling Pathways. Cancer Letters, 355, 232-241.
http://dx.doi.org/10.1016/j.canlet.2014.08.037

[4] Xu, W.R., Lin, H.S., Yang, L.L., et al. (2011) Compound Kushen Injection Suppresses Human Breast Cancer Stem- Like Cells by Down-Regulating the Canonical Wnt/β-Catenin Pathway. Experimental & Clinical Cancer Research, 30, 103.
http://dx.doi.org/10.1186/1756-9966-30-103

[5] 马悦. 复方苦参注射液化学成分及质量控制研究[D]: [硕士学位论文]. 北京: 中国中医科学院, 2012: 1-25.

[6] 关扎根, 吴尚英, 李安平, 等. 短柱肖菝葜的资源调查及品质分析[J]. 中国现代中药, 2014, 16(6): 444-446.

[7] 秦文杰, 王钢力, 林瑞超. 短柱肖菝葜化学成分的研究[J]. 中草药, 2007, 38 (10): 1466-1468.

[8] 蒯玉花, 海丽娜, 菅晓勇, 等. 肖菝葜属药用植物的研究进展[J]. 中国实验方剂学杂志, 2010, 16(11): 207-209.

[9] Jerald, J.N. and Johannes, V.S. (2012) Isolation and Quantification of the Toxic Methylazoxymethanol Glycoside Macrozamin in Selected South African Cycad Species. South African Journal of Botany, 82, 108-112.
http://dx.doi.org/10.1016/j.sajb.2012.07.011

[10] Nishida, K., Kobayashi, A. and Nagahama, T. (1955) Cycasin, a New Toxic Glycoside of Cycas revoluta Thunb. I. Isolation and Structure of Cycasin. Bulletin of the Agricultural Chemical Society of Japan, 19, 77-84.

[11] Xu, H.X., Kadota, S., Wang, H., et al. (1994) A New Hydrolyzable Tannin from Geum Japonicum and Its Antiviral Activity. Heterocycles, 38, 167-175.
http://dx.doi.org/10.3987/COM-93-6550

[12] 张雪梅, 李磊, 张刚磊, 等. 3,4-二羟基苯甲醛合成工艺的改进[J]. 徐州师范大学学报, 2006, 24(3): 51-53.

[13] 毛士龙, 廖时萱, 吴久鸿, 等. 黄花远志根化学成分的分离和结构鉴定[J]. 药学学报, 1996, 31(2): 118-121.

[14] 张金超, 申勇, 朱国元, 等. 杜鹃兰的化学成分研究[J]. 中草药, 2007, 38(8): 1161-1162.

[15] Marianna, H., Jean, F.B., Mourad, K., et al. (1988) Monohydroxy- and 2,5-Dihydroxy Terephthalic Acids, Two Unusual Phenolics Isolated from Centaurium erythraea and Identified in Other Gentianaceae Members. Journal of Natural Products, 51, 977-980.
http://dx.doi.org/10.1021/np50059a030

[16] Zhao, Y. and Ruan, J.L. (2006) Chemical Constituents from Piper wallichii. Journal of Chinese Pharmaceutical Sciences, 15, 21-23.

[17] Ripperger, H., Diaz, M. and Schreiber, K. (1981) Two Cinnamoyl Derivatives from Cinnamomum tripli-nervis. Phytochemistry, 20, 1453-1454.
http://dx.doi.org/10.1016/0031-9422(81)80070-2

[18] Nishizawa, Y. and Kodama, T. (1966) Homopantothenic Acid. Bitamin, 33, 589-602.

[19] Yumrutas, O., Sokmen, A., Akpulat, H.A., et al. (2012) Phenolic Acid Contents, Essential Oil Compositions and Antioxidant Activities of Two Varieties of Salvia Euphratica from Turkey. Natural Product Research, 26, 1848-1851.
http://dx.doi.org/10.1080/14786419.2011.613386

[20] Smashevskii, N.D., Kopelevich, V.M., Marieva, T.D., et al. (1973) Antivitamin Action of D-, L-, and DL-Homopan- tothenic Acids on Yeasts. Applied Biochemistry & Micro-biology, 9, 659-663.

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