GSK-3β对流感病毒在A549细胞中复制的影响
The Impact of GSK-3β on the Replication of Influenza Virus in A549 Cells
作者: 戴新宪 ：北京生物制品研究所有限责任公司，北京; 张丽姝 , 韩鸿雅 ：北京交通大学理学院，北京;
关键词: 病毒学； 流感病毒； 细胞凋亡； 糖原合成激酶-3β； 病毒与宿主相互作用； Virology； Influenza Virus； Cell Apoptosis； Glycogen Synthase Kinase-3β； Viral-Host Interaction
摘要:Abstract: Objective: In order to clarify the role of glycogen synthase kinase-3β (GSK-3β) in the process of influenza virus infection. Methods: We preformed the RNAi to knock down the GSK-3β gene expression, then we determined the virus titers in the A549 cell supernatants by performing TCID 50 assay, and examined the influence of GSK-3β to cell viabilities of the infected cells as well as the influenza virus-induced A549 cell apoptosis using CCK-8 Kit and Caspase-Glo 3/7 Kit. Results: The results demonstrated that down-regulation of GSK3 expression decreased the viral titers in the A549 cells supernatants. Down-regulation of GSK-3β expression decreased the A549 cell viability, at the same time, increased the influenza virus induced cell apoptosis. Conclusion: Hence, GSK-3β negatively regulated the influenza virus-induced host cell apoptosis, and it was essential for the efficient replication of influenza virus.
Abstract:
文章引用: 戴新宪 , 张丽姝 , 韩鸿雅 (2016) GSK-3β对流感病毒在A549细胞中复制的影响。 自然科学， 4， 371-377. doi: 10.12677/OJNS.2016.44045
参考文献
[1]
Embi, N., Rylatt, D.B. and Cohen, P. (1980) Glycogen Synthase Kinase-3 from Rabbit Skeletal Muscle. Separation from Cyclic-AMP-Dependent Protein Kinase and Phosphorylase Kinase. European Journal of Biochemistry, 107, 519- 527.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb06059.x
[2]
Rylatt, D.B., Aitken, A., Bilham, T., Condon, G.D., Embi, N. and Cohen, P. (1980) Glycogen Synthase from Rabbit Skeletal Muscle. Amino Acid Sequence at the Sites Phosphorylated by Glycogen Synthase Kinase-3, and Extension of the N-Terminal Sequence Containing the Site Phosphorylated by Phosphorylase Kinase. European Journal of Biochemistry, 107, 529-537.
http://dx.doi.org/10.1111/j.1432-1033.1980.tb06060.x
[3]
Jope, R.S. and Johnson, G.V. (2004) The Glamour and Gloom of Glycogen Synthase Kinase-3. Trends in Biochemical Sciences, 29, 95-102.
http://dx.doi.org/10.1016/j.tibs.2003.12.004
[4]
Beurel, E. and Jope, R.S. (2006) The Paradoxical Pro- and An-ti-Apoptotic Actions of GSK3 in the Intrinsic and Extrinsic Apoptosis Signaling Pathways. Progress in Neurobiology, 79, 173-189.
http://dx.doi.org/10.1016/j.pneurobio.2006.07.006
[5]
Chin, P.C., Majdzadeh, N. and D’Mello, S.R. (2005) Inhibition of GSK3β Is a Common Event in Neuroprotection by Different Survival Factors. Molecular Brain Research, 137, 193-201.
http://dx.doi.org/10.1016/j.molbrainres.2005.03.004
[6]
Shin, S.Y., Kim, C.G., Jho, E.-H., Rho, M.-S., Kim, Y.S., Kim, Y.-H. and Lee, Y.H. (2004) Hydrogen Peroxide Negatively Modulates Wnt Signaling through Downregulation of β-Catenin. Cancer Letters, 212, 225-231.
http://dx.doi.org/10.1016/j.canlet.2004.03.003
[7]
Srinivasan, S., Ohsugi, M., Liu, Z., Fatrai, S., Bernal-Mizrachi, E. and Permutt, M.A. (2005) Endoplasmic Reticulum Stress-Induced Apoptosis Is Partly Mediated by Reduced Insulin Signaling through Phosphatidylinositol 3-Kinase/Akt and Increased Glycogen Synthase Kinase-3β in Mouse Insulinoma Cells. Diabetes, 54, 968-975.
http://dx.doi.org/10.2337/diabetes.54.4.968
[8]
Watcharasit, P., Bijur, G.N., Song, L., Zhu, J., Chen, X. and Jope, R.S. (2003) Glycogen Synthase Kinase-3β (GSK3β) Binds to and Promotes the Actions of p53. The Journal of Biological Chemistry, 278, 48872-48879.
http://dx.doi.org/10.1074/jbc.M305870200
[9]
Watcharasit, P., Bijur, G.N., Zmijewski, J.W., Song, L., Zmijewska, A., Chen, X., Johnson, G.V.W. and Jope, R.S. (2002) Direct, Activating Interaction between Glycogen Synthase Kinase-3β and p53 after DNA Damage. Proceedings of the National Academy of Sciences of the United States of America, 99, 7951-7955.
http://dx.doi.org/10.1073/pnas.122062299
[10]
Song, L., Zhou, T. and Jope, R.S. (2004) Lithium Facilitates Apoptotic Signaling Induced by Activation of the Fas Death Domain-Containing Receptor. BMC Neuroscience, 5, 1-7.
http://dx.doi.org/10.1186/1471-2202-5-20
[11]
Bournat, J.C., Brown, A.M.C. and Soler, A.P. (2000) Wnt-1 Dependent Activation of the Survival Factor NF-κB in PC12 Cells. Journal of Neuroscience Research, 61, 21-32.
http://dx.doi.org/10.1002/1097-4547(20000701)61:1<21::AID-JNR3>3.0.CO;2-7
[12]
Ciani, L. and Salinas, P.C. (2005) WNTs in the Vertebrate Nervous System: From Patterning to Neuronal Connectivity. Nature Reviews. Neuroscience, 6, 351-362.
http://dx.doi.org/10.1038/nrn1665
[13]
Zmijewski, J.W. and Jope, R.S. (2004) Nuclear Accumulation of Glycogen Synthase Kinase-3 during Replicative Senescence of Human Fibroblasts. Aging Cell, 3, 309-317.
http://dx.doi.org/10.1111/j.1474-9728.2004.00117.x
[14]
Ehrhardt, C., Wolff, T., Pleschka, S., Planz, O., Beermann, W., Bode, J.G., Schmolke, M. and Ludwig, S. (2007) Influenza A Virus NS1 Protein Activates the PI3K/Akt Pathway to Mediate Antiapoptotic Signaling Responses. Journal of Virology, 81, 3058-3067.
http://dx.doi.org/10.1128/JVI.02082-06