金果榄药材的UPLC指纹图谱研究
Study on UPLC Fingerprint of Tinospora sagittata and T. capillipes

作者: 李扬华 , 陈秋虹 , 梁兰娜 , 徐 慧 , 蒋艳芳 :广西分析测试研究中心,广西 南宁 ;

关键词: 金果榄UPLC指纹图谱质量控制 Tinospora sagittata and T. capillipes UPLC Fingerprints Quality Control

摘要:
目的:建立金果榄药材的UPLC指纹图谱,为金果榄药材的质量控制提供依据。方法:采用超高效液相色谱法。色谱条件:色谱柱:Acquity UPLC®BEN C18 (2.1 × 100 mm,1.7 µm);流动相A:乙腈;流动相B:0.3%磷酸溶液;流速:0.25 ml/min;检测波长:225 nm;柱温:25℃。结果:建立了10批金果榄药材的指纹图谱。金果榄药材有7个共有峰,多数峰可以达到较好分离,具有较高的相似度。结论:建立的超高效液相指纹图谱有较好的精密度、重复性和稳定性,可作为金果榄质量评价参考。

Abstract: Objective: To establish UPLC fingerprint of Tinospora sagittata and T. capillipes which is expected to provide standards for the quality control and identification of the Chinese crude drug. Method: The UPLC method was used. Chromatography conditions were Acquity UPLC®BEN C18 (2.1 × 100 mm, 1.7 µm) column with gradient mobile phase of acetonitrile and 0.3% phosphate acid. UV detection wavelength was 225 nm, the flow rate was 0.25 ml/min and the column temperature was 25˚C. Result: UPLC fingerprints of 10 samples of Tinospora sagittata and T. capillipes were established. 7 mutual peaks were selected as the fingerprint peaks in the samples. Among the obtained fingerprints, most of the detected peaks were separated effectively. 10 samples had higher simi-larities. Conclusion: The established UPLC fingerprint has desirable accuracy, repeatability and sta-bility, which can be used as reference for the quality control of Tinospora sagittata and T. capillipes.

文章引用: 李扬华 , 陈秋虹 , 梁兰娜 , 徐 慧 , 蒋艳芳 (2016) 金果榄药材的UPLC指纹图谱研究。 分析化学进展, 6, 79-85. doi: 10.12677/AAC.2016.64012

参考文献

[1] 国家药典委员会. 中华人民共和国药典[S]. 北京: 化学工业出版社, 2010: 202.

[2] 杨宏, 王天志, 杜蕾蕾, 等. RP-HPLC测定金果榄药材中巴马汀和药根碱的含量[J]. 华西药学杂志, 2004, 19(3): 195-196.

[3] 杨光义, 杜士明, 黄良永, 等. 不同产地金果榄药材中盐酸巴马汀含量比较[J]. 医药导报, 2008, 27(9): 1116- 1117.

分享
Top