The Expression of Inflammatory Cytokines in Liver Cirrhosis Rats Serum under the Condition of the Whole Liver Blood Flow Blocking
Abstract: Objective: To research the expression of inflammatory cytokines which easily lead to multiple organ damage in liver cirrhosis rats serum under the condition of the whole liver blood flow blocking. Methods: Eights male SD rats weight 160 - 200 g were selected. The liver cirrhosis rats model was established by 30% CCl4 solution to fill the stomach and 5% alcohol to feed. Eighty SD rats were randomly divided into four groups, with 20 rats in each group. By blocking porta hepatis, liver up and inferior vena cava for 15 min (A2 group ), 30 min (A3group), 45 min (A4 group) production of cirrhosis rats whole liver blood flow blocking model, and the sham operation group (A1 group) as control. Twenty rats in each group were sacrificed at 15, 30, 45 min, respectively after modeling. According to the blocking time, they were killed lively, to monitor the expression of TNF-α, NF-KB and IL-21 in the serum from the inferior vena cava. All data were analyzed by using the analysis of variance or t test. Results: Cirrhosis rats whole liver blood flow blocking model compared with those in group A1, levels of TNF-α, NF-KB and IL-21 were significantly increased (p < 0.05), and the expression of inflammatory cytokines trend to grow by time. Conclusion: The expression of inflammatory cytokines which easily lead to multiple organ damage in liver cirrhosis rats serum under the condition of the whole liver blood flow blocking was increased. The concentration of inflammatory cytokines increases with the extension of blocking time. There is a mutual influence among different inflammatory cytokines which play a role in the injury of organ function.
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