Effects of Phosphate on the Growth and Cell Pigment Accumulation of Dunaliella salina
Abstract: The effects of KH2PO4 with different levels on the growth and cell pigment accumulation of Dunaliel-la salina were investigated and the results indicated that the maximum growth of 153 × 104 cell/mL was detected in culture medium with 0.1 mmol/L KH2PO4 and the minimum of 36 × 104 cell/mL in the control group. The culture medium with 0.20 mmol/L KH2PO4 had the highest β-carotene con-tent of 12.8 mg/L, followed by 11.04 mg/L with 0.15 mmol/L KH2PO4, and the control group had lower β-carotene content of only 6.28 mg/L. Over 0.25 mmol/L KH2PO4 was found to have depress-ing function to cell growth. Right level KH2PO4 was helpful to chlorophyll a biosynthesis. During the first 6 days within culture period, pH values of the culture media rose up, and then declined in the following 4 - 5 days. The cells which were cultured in media with 0.10 mmol/L KH2PO4 had the high-est protein content of 37.34%, while the control group only had 24.86%; 0.10 mmol/L KH2PO4 was assimilated completely within 6 days, and the uptake time had right-relationship with KH2PO4 con-tents. Finally, the assimilated mechanic equation of KH2PO4 by D. salina was set up.
文章引用: 刘笑天 , 王培磊 (2016) 磷酸盐对盐生杜氏藻生长和细胞色素积累的影响。 海洋科学前沿， 3， 100-107. doi: 10.12677/AMS.2016.33014
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