分子粪便学在中缅树鼩中的应用可能性
Application of Molecular Scatology in Tree Shrews, Tupaia belangeri

作者: 严毅 , 陈金龙 * , 何银忠 :昆明市海口林场,云南 昆明; 王娟 :云南师范大学文理学院,云南 昆明; 付家豪 :云南师范大学生命科学学院,云南 昆明;

关键词: 分子粪便学非损伤性取样中缅树鼩Molecular Scatology Non Invasive Sampling Tupaia belangeri

摘要: 中缅树鼩(Tupaia belangeri)作为东洋界特有的小型哺乳动物,主要处于横断山山脉地区,其已被证实与灵长目亲缘性较近,并逐渐在医学上被应用为实验动物,但是对于其野外的研究甚少。本文以分子粪便学为基础从分子标记、性别鉴定以及巢域确定等方面阐述了其应用在野外中缅树鼩研究中的可能性,为进一步研究中缅树鼩及使其成为标准实验动物化化与合适的实验动物疾病模型提供了理论依据。

Abstract: Tree shrew (Tupaia belangeri) as the small mammals in oriental endemic, mainly in Hengduan Mountains region, has been proved to be close affinity with the primates, and gradually became an experimental animal. But the study of the wild is very little. This paper, based on molecular sca-tology, expounds the possibility of molecular scatology being employed in the study of Tupaia belangeri in wild area in these aspects of molecular marker, sex determination and home range con-firmation. It lays a foundation for the further study in Tupaia belangeri, making it become the standard experimental animals and an appropriate experimental animal disease model provides a theoretical basis.

文章引用: 严毅 , 陈金龙 , 何银忠 , 王娟 , 付家豪 (2016) 分子粪便学在中缅树鼩中的应用可能性。 生物过程, 6, 58-63. doi: 10.12677/BP.2016.63009

参考文献

[1] 王应祥, 李崇云, 马世来. 树鼩的分类与生态. 树鼩生物学[M]. 昆明: 云南科技出版社, 1991: 21-70.

[2] Frantzen, M.A.J., Silk, J.B., Ferguson, J.W.H., et al. (1998) Empirical Evaluation of Preservation Methods for Faecal DNA. Molecular Ecology, 7, 1423-1428.
http://dx.doi.org/10.1046/j.1365-294x.1998.00449.x

[3] Piggott, M.P. and Taylor, A.C. (2003) Extensive Evalution of Faecal Preservation and DNA Extraction Methods in Australian Native and Introduced Species. Australian Journal of Zoology, 51, 341-355.
http://dx.doi.org/10.1071/ZO03012

[4] Flagstad, R.K., Stacy, J.E., et al. (1999) Reliable Noninvasive Genotyping Based on Excremental PCR of Nuclear DNA Purified with a Magneric Bead Protocol. Molecular Ecology, 8, 879-883.
http://dx.doi.org/10.1046/j.1365-294X.1999.00623.x

[5] Reed, J.Z., Tollit, D.J., Thompson, P.M. and Amos, W. (1997) Mo-lecular Scatology: The Use of Molecular Genetic Analysis to Assign Species, Sex and Individual Identity to Seal Faeces. Molecular Ecology, 6, 225-234.
http://dx.doi.org/10.1046/j.1365-294X.1997.00175.x

[6] 钟华, 赖旭龙, 魏荣平, 等. 一种从大熊猫粪便中提取DNA的改进方法[J]. 四川动物, 2003(49): 670-674.

[7] Avise, J.C., Nesson, W.S. and Sibley, C.G. (1994) DNA Sequence Support for Class Phylogenetic Relationships between Some Storks and New World Vultures. Proceedings of the National Academy of Science of the USA, 91, 5173- 5177.
http://dx.doi.org/10.1073/pnas.91.11.5173

[8] Nakamura, Y., et al. (1987) Variable Number of Tandem Repeat (VNTR) Markers for Human Gene Mapping. Science, 235, 1616-1622.
http://dx.doi.org/10.1126/science.3029872

[9] 方盛国, 陈冠群, 冯文和, 张安居, 李绍昌, 何光昕, 魏辅文, 郭键. 大熊猫DNA指纹在野生种群数量调查中的应用[J]. 兽类学报, 1996(4): 246-249.

[10] 方盛国, 冯文和, 张安居, 余健秋, 李学兵, 黄祥明, 费立松, 陈红卫, 张志和, 张坚, 雍严格. 佛坪三官庙地区大熊猫种群数量的DNA指纹分析[J]. 四川大学学报, 1997, 34(专辑): 104-107.

[11] Sinclair, A.H., et al. (1990) A Gene from the Human Sex-Determining Region Encodes a Protein with Homology to a Conserved DNA-Binding Motif. Nature, 346, 240-245.
http://dx.doi.org/10.1038/346240a0

[12] 王加连, 杨光, 周开亚, 魏辅文, 严洁. PCR扩增Sry基因进行鲸类动物性别的鉴定[J]. 兽类学报, 2005, 25(1): 20-23.

[13] Reed, J.Z., Tollit, D.J., Thompson, P.M., et al. (1997) Molecular Scatology: The Use of Molecular Genetic Analysis to Assign Species, Sex and Individual Identity to Seal Faeces. Molecular Ecology, 6, 225-234.
http://dx.doi.org/10.1046/j.1365-294X.1997.00175.x

[14] Kohn, M., Knauer, F., Stoffella, A., Schröder, W. and Pääbo, S. (1995) Conservation Genetics of the European Brown Bear—A Study Using Excremental PCR of Nuclear and Michondrial Sequences. Molecular Ecology, 4, 95-103.
http://dx.doi.org/10.1111/j.1365-294X.1995.tb00196.x

[15] Kohn, M.H. and Wayne, R.K. (1997) Facts from Feces Revisited. Trends in Ecology & Evolution, 12, 223-227.
http://dx.doi.org/10.1016/S0169-5347(97)01050-1

分享
Top