The Effects of Rapeseed Oil and Linseed Oil on Biomass and Metabolites of Lachnum sp.
Abstract: The research mainly investigated the effects of different concentrations of rapeseed oil and linseed oil on biomass and metabolites of Lachnum sp. Different concentrations of 0.5%, 1%, 2%, 3% and 4% rapeseed oil or linseed oil were added into culture medium respectively and fermented for 10 days, it was found that the biomass of 1% rapeseed oil and 2% linseed oil were 4.26 g/L and 4.27 g/L, which were almost equal to that absence of lipids (4.29 g/L), while the biomass with rapeseed oil and linseed oil of other concentrations was inhibited at different degrees. However, there was no significant difference in the biomass of the same oil among different concentrations. Rapeseed oil and linseed oil in different concentrations could inhibit exopolysaccharides (LEP) production significantly. The fermentation broth with 1% rapeseed oil and 2% linseed oil was selected, then metabolites in chloroform and ethyl acetate extraction components of fermentation broth and the original one were analyzed by Liquid Chromatograph Mass Spectrometer (LC-MS), the results showed that 27 kinds of substances were detected in the fermentation broth with 1% rapeseed oil and 20 kinds of substances were detected in the one with 2% linseed oil, only 4 same substances were detected in the fermentation broth added with these two kinds of lipids, which suggested that different kinds of lipids had significant influences on metabolites. 3 same substances were detected in the fermentation broth with 1% rapeseed oil after extracted with two extraction solvents, while 4 same substances were detected in the fermentation broth (with 2% flax seed oil) extractions, which illustrated that the test results of different broth extractions were obviously different. The results of the research indicate that different kinds of lipids have inhibiting effects on biomass and LEP production of Lachnum and the inhibition effects of different concentrations of oil are different; different kinds of lipids can affect the types of metabolites of Lachnum.
文章引用: 宗 帅 , 赵墨元 , 李井雷 , 黄 曼 , 杨柳青 , 叶 明 (2016) 菜籽油和亚麻籽油对粒毛盘菌生物量和代谢产物的影响。 微生物前沿， 5， 26-36. doi: 10.12677/AMB.2016.53004
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