Study on Taiwan Wentan and Miyu Pummeloes Limonoid Glucosyltransferase
Abstract: Influence of limonin glusocyltransferease displays much influence on citrus juice bitterness, espe-cially in pumeloes. In this study, we have cloned a 1600 bp lgt from pumeloes limonin glucosyl-transferase’s active site, a 44 amino acids open reading frame was determined. This opening reading frame would be useful for genetic construction and cloning in upcoming study for citrus juice enhancement in flavor. This study also investigated Wentan and Miyu lgt during flowering and maturing. The results indicated that type II lgt was found only in Miyu and the gene expression was actively found in fruit. Wentan have both type I and type II lgt but the expression level is much lower than Miyu. These findings were similar to past researches which indicated that matured Wentan had less lgt expression in their fruit thus much bitter compounds appearance during its processing. We also used FPLC to purify LGTase, an approximately 40 kDa protein was then obtained and quantitated at 0.6 and 1 mg/ml. The enzyme can be used to reduce bitterness in pummeloes and further increase utility of peel wastes in processing.
文章引用: 吴思节 , 徐源泰 (2016) 台湾文旦柚和蜜柚之柠檬苦素葡萄糖转移酶研究。 农业科学， 6， 99-109. doi: 10.12677/HJAS.2016.64015
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