Preparation of BMP-2-PLLA Microspheres and Its Maintenance for Bioactivity of BMP-2
Abstract: The preparation conditions of poly-l-lactide (PLLA) microspheres, release characteristics and bi-oactivity of Bone Morphogenetic Protein-2 (BMP-2) were studied. Bovine serum albumin (BSA)- loaded PLLA microspheres were prepared with a double emulsion method. The effects of the mo-lecular weight and concentration of PLLA, the molecular weight of PEG on the particle size, loading capacity and entrapment efficiency were studied. Then, the optimal conditions were selected to prepare BMP-2-loaded PLLA microspheres, and the bioactivity of BMP-2 was investigated by pro-duction of cell Alkaline Phosphatase (ALP). The results showed that the BMP-2-PLLA microspheres were spherical shape with diameters of 4.815 μm. The encapsulation efficiency of the BMP-2 in PLLA microspheres was 70.88%, and the loading capacity was 2.28 × 10−3%. The residual amount of methylene chloride in BMP-2-PLLA microsphere was 0.0041%. BMP-2-loaded PLLA microspheres had excellent drug sustained-release performance, which would control release BMP-2. BMP-2 biological activity indicated that the biological activity was not effected after emulsion process. The bioactivity of growth factors released from PLLA microspheres was more durable and effective. PLLA microspheres prepared can be used to load growth factors to control their release and maintain their biological activity.
文章引用: 白 燕 , 肖 唯 (2016) BMP-2-PLLA缓释微球的制备及其对BMP-2活性的维持。 材料科学， 6， 268-275. doi: 10.12677/MS.2016.64035
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