Preparation and Release Performance in Vitro of Chitosan Microspheres/nHA/PLGA Compound Scaffolds
Abstract: In order to construct a bone regeneration scaffold that different growth factors controlled release at proper stage, Trypsin (Try) was selected as the model protein. The composite of sustained-re- lease microspheres and biodegradable porous scaffolds was prepared in this study. Firstly, Tryp-sin-chitosan microspheres (CMs) were prepared. Then, Trypsin-CMs were compounded to the nano hydroxyapatite/poly lactic-co-glycolic acid (nHA/PLGA) to build a scaffold that could release growth factors sequentially. The results showed that the Try-CMs were spherical shape with diameters of 4 - 10 μm. The encapsulation efficiency of the Trypsin in CMs was 61.33%, and the loading capacity was 25.699%. The prepared Try-CMs/nHA/PLGA scaffold possessed 100 - 200 μm pore diameter, 53.24% porosity, 7.31 MPa compressive strength, and 19.92% degradation at 8 weeks. The cumulative releases of Trypsin from Try-nHA/PLGA, Try-CMs and Try-CMs/nHA/PLGA scaffolds were respectively about 57.31%, 69.32% and 26.03% at 48 hours, 77.89%, 85.73% and 54.53% at 14 days. The results demonstrated that Try-CMs/nHA/PLGA scaffolds had excellent drug sustained-release performance, which would be used as tissue engineering scaffolds with protein controlled delivery.
文章引用: 白 燕 , 肖 唯 (2016) CMs/nHA/PLGA复合支架的制备及体外释放性能的研究。 材料科学， 6， 256-262. doi: 10.12677/MS.2016.64033
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