各期糖尿病视网膜病变患者房水中PEDF与OPs的相关性分析
Aqueous Humor Levels of Pigment Epithelium-Derived Factor and Oscillatory Potentials in Diabetic Retinopathy Patients

作者: 臧 晶 , 王文娟 , 鲍炯琳 , 陈立伦 :广东药学院附属第一医院,广东 广州; 管国奇 :暨南大学,广东 广州;

关键词: 糖尿病视网膜病变PEDFOPsDiabetic Retinopathy Pigment Epithelium Derived Factor Oscillatory Potentials

摘要:
目的:探讨房水中色素上皮衍生因子(pigment epithelium derived factor, PEDF)与视网膜电图振荡电位(oscillatory potentials, OPs)在各期糖尿病性视网膜病变中的相关性研究分析,为糖尿病视网膜病变(diabetic retinopathy, DR)的早期诊断及病程进展预测提供客观的检测依据。方法:房水来自2型糖尿病患者40例40眼。单纯2型糖尿病患者组19例19只眼,非增殖期糖尿病性视网膜病变(non-proliferative diabetic retinopathy, NPDR)组患者11例11只眼,增殖期糖尿病视网膜病变(proliferative diabetic retinopathy, PDR)组患者10例10只眼,单纯老年性白内障患者20例20只眼作为对照组。用酶联免疫吸附法检測房水中PEDF的水平,用德国 ROLAND CONSULT公司生产的罗兰视觉电生理检查仪检测全视网膜电图中OPs的总振幅值。结果:单纯2型糖尿病患者组、NPDR组及PDR组的房水中PEDF的浓度分别为(9.13 ± 4.43) ng/ml、(7.00 ± 2.04) ng/ml、(6.39 ± 1.66) ng/ml与对照组(13.87 ± 0.36) ng/ml相比,房水中PEDF浓度均明显降低(P < 0.01),单纯2型糖尿病患者组与NPDR组及PDR组相比,房水中PEDF浓度亦均明显降低(P = 0.04, P = 0.02),而NPDR组与PDR组相比无明显差异(P = 0.34);单纯2型糖尿病患者组、NPDR组及PDR组的OPs的总振幅值分别为(164.56 ± 37.41) μV、(79.80 ± 19.89) μV、(28.55 ± 17.47) μV与对照组(237.96 ± 35.96) μV相比,OPs的总振幅值均明显降低(P < 0.01),而且组间两两比较亦有明显差异(P < 0.01);各期糖尿病性视网膜病变房水中PEDF浓度变化与OPs的总振幅值呈典型的正相关(r = 0.905, P < 0.01)。结论:房水中PEDF的浓度与OPs总波幅在各期糖尿病性视网膜病变中呈正相关,可为糖尿病视网膜病变的早期诊断及病程进展预测提供客观的检测依据。

Abstract: Objective: To investigate the relationship between pigment epithelium derived factor (PEDF) and oscillatory potentials (OPs) in various diabetic retinopathy and to provide an objective basis for the early diagnosis of diabetic retinopathy (diabetic retinopathy, DR) and the prediction of the progression of the disease. Methods: Aqueous humor samples were collected from 40 eyes of 40 type 2 diabetes patients, including 19 eyes without diabetic retinopathy and 21 eyes with diabetic (11 eyes with NPDR and 10 eyes with PDR), twenty aqueous samples from 20 cataract patients without other ocular or systemic diseases served as controls. The PEDF levels in aqueous humor were measured by enzyme-linked immunosorbent assay (ELISA). The total amplitude of OPs in the whole retina was detected by the Roland visual electrophysiological test instrument of the German CONSULT ROLAND company. Results: The PEDF and OPs level in eyes without diabetic retinopathy [(9.13 ± 4.43) ng/ml, 164.56 ± 37.41) μV, P < 0.01] was significantly lower than that in controls [(13.87 ± 0.36) ng/ml, (237.96 ± 35.96) μV]; the PEDF Ievel in eyes with NPDR [(7.00 ± 2.04) ng/ml, (79.80 ± 19.89) μV, P < 0.01] and PDR [(6.39 ± 1.66) ng/ml, (28.55 ± 17.47) μV, P < 0.01] was sig-nificantly lower than that in controls [(13.87 ± 0.36) ng/ml, (237.96 ± 35.96) μV]; the concentration of PEDF in the patients with type 2 diabetes mellitus was significantly lower than that in the NPDR and PDR group (P = 0.04, P = 0.02), while there was no significant difference between NPDR group and PDR group (P = 0.34). There were significant differences in different OPs group (P < 0.01). The PEDF concentration in the aqueous humor of the diabetic retinopathy was a typical positive correlation with the total amplitude of OPs (r = 0.905, P < 0.01). Conclusion: The concentration of PEDF in aqueous humor and the total amplitude of OPs were positively correlated with the diabetic retinopathy, which could provide an objective basis for the early diagnosis of diabetic retinopathy and the prediction of the progression of the disease.

文章引用: 臧 晶 , 管国奇 , 王文娟 , 鲍炯琳 , 陈立伦 (2016) 各期糖尿病视网膜病变患者房水中PEDF与OPs的相关性分析。 眼科学, 5, 22-28. doi: 10.12677/HJO.2016.51005

参考文献

[1] 霍鸣, 张海江, 吴昊, 等. 糖尿病视网膜病变的激光光凝治疗[J]. 国际眼科杂志, 2007, 7(1): 202-203.

[2] Cantrill, H.L. (1984) The Diabetic Retinopathy Study and the Early Treatment Diabetic Retinopathy Study. International Ophthalmology Clinics, 24, 13-29.
http://dx.doi.org/10.1097/00004397-198402440-00004

[3] Josifova, T., Schneider, U., Henrich, P.B. and Schrader, W. (2008) Eye Disorders in Diabetes: Potential Drug Targets. Infectious Disorders: Drug Targets, 8, 70-75.
http://dx.doi.org/10.2174/187152608784746529

[4] Boehm, B.O., Lang, G., Feldmann, B., Kurkhaus, A., Ro-singer, S., Volpert, O., et al. (2003) Proliferative Diabetic Retinopathy Is Associated with a Low Level of the Natural Ocular Anti-Angiogenic Agent Pigment Epithelium-Derived Factor (PEDF) in Aqueous Humor. A Pilot Study. Hor-mone and Metabolic Research, 35, 382-386.
http://dx.doi.org/10.1055/s-2003-41362

[5] 郭秀瑾, 王长龄, 张怡红, 等. 视网膜振荡电位在糖尿病性视网膜病变早期诊断的价值[J]. 河北医科大学学报, 2002(23): 14-18.

[6] 叶任高, 陆再英. 内科学[M]. 6版. 北京: 人民卫生出版社, 2004, 5-797.

[7] Sandri, F., Ancora, G., Lanzoni, A., et al. (2004) Prophylactic Nasal Continuous Positive Airways Pressure in Newborns of 28 - 31 Weeks Gestation: Multicentre Randomised Controlled Clinical Trial. Archives of Disease in Childhood—Fetal and Neonatal Edition, 89, F394-F398.
http://dx.doi.org/10.1136/adc.2003.037010

[8] Hentschel, R. and Jorch, G. (2002) Acute Side Effects of Surfac-tant Treatment. Journal of Perinatal Medicine, 30, 143-148.

[9] Nam, D.H., Oh, J., Roh, J.H. and Huh, K. (2009) Different Expression of Vascular Endothelial Growth Factor and Pigment Epithelium-Derived Factor between Diabetic and Non-Diabetic Epiretinal Membranes. Ophthalmologica, 223, 188-191.
http://dx.doi.org/10.1159/000198686

[10] Ogata, N., Nishikawa, M., Nishimura, T., Mitsuma, Y. and Matsumura, M. (2002) Unbalanced Vitreous Levels of Pigment Epithelium-Derived Factor and Vascular Endothelial Growth Factor in Diabetic Retinopathy. American Journal of Ophthalmology, 134, 348-353.
http://dx.doi.org/10.1016/S0002-9394(02)01568-4

[11] Matsuoka, M., Ogata, N., Minamino, K. and Matsumura, M. (2007) Leukostasis and Pigment Epithelium-Derived Factor in Rat Models of Diabetic Retinopathy. Molecular Vision, 13, 1058-1065.

[12] 陈海冰, 贾伟平, 陆俊茜, 包玉倩, 等. 2型糖尿病肾病患者血清色素上皮源因子水平的变化及意义[J]. 中华医学杂志, 2007, 87(18): 1230-1233.

[13] Boehm, B.O., Lang, G., Volbert, O., Jehle, P.M., Kurkhaus, A., Rosinger, S., et al. (2003) Low Content of the Natural Ocular Anti-Angiogenic Agent Pigment Epithe-lium-Derived Factor (PEDF) in Aqueous Humor Predicts Progression of Diabetic Retinopathy. Diabetologia, 46, 394-400.

[14] Yamagishi, S., Matsui, T., Nakamura, K., Takeuchi, M. and Imaizumi, T. (2006) Pigment Epithe-lium-Derived Factor (PEDF) Prevents Diabetes- or Advanced Glycation End Products (AGE)-Elicited Retinal Leukos-tasis. Microvascular Research, 72, 86-90.

[15] Bearse Jr., M.A., Shimada, Y. and Sutter, E.E. (2000) Distribution of Oscillatory Components in the Central Retina. Documenta Ophthalmologica, 100, 185-205.
http://dx.doi.org/10.1023/A:1002783719958

[16] Kunikata, H., Nakagawa, Y. and Tamai, M. (2004) Evaluation of Visual Function and Prognosis for Patients with Proliferative Diabetic Retinopathy with the Low Vision Evaluator. The Tohoku Journal of Experimental Medicine, 204, 229-236.
http://dx.doi.org/10.1620/tjem.204.229

[17] Leozappa, M., Micelli Ferrari, T., Grossi, T., Pace, V., Rinaldi, M.L., Battista, D. and Micelli Ferrari, L. (2008) Prognostic Prediction Ability of Postoperative Multifocal ERG after Vitrectomy for Diabetic Macular Edema. European Journal of Ophthalmology, 18, 609-613.

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