Aqueous Humor Levels of Pigment Epithelium-Derived Factor and Oscillatory Potentials in Diabetic Retinopathy Patients
Abstract: Objective: To investigate the relationship between pigment epithelium derived factor (PEDF) and oscillatory potentials (OPs) in various diabetic retinopathy and to provide an objective basis for the early diagnosis of diabetic retinopathy (diabetic retinopathy, DR) and the prediction of the progression of the disease. Methods: Aqueous humor samples were collected from 40 eyes of 40 type 2 diabetes patients, including 19 eyes without diabetic retinopathy and 21 eyes with diabetic (11 eyes with NPDR and 10 eyes with PDR), twenty aqueous samples from 20 cataract patients without other ocular or systemic diseases served as controls. The PEDF levels in aqueous humor were measured by enzyme-linked immunosorbent assay (ELISA). The total amplitude of OPs in the whole retina was detected by the Roland visual electrophysiological test instrument of the German CONSULT ROLAND company. Results: The PEDF and OPs level in eyes without diabetic retinopathy [(9.13 ± 4.43) ng/ml, 164.56 ± 37.41) μV, P < 0.01] was significantly lower than that in controls [(13.87 ± 0.36) ng/ml, (237.96 ± 35.96) μV]; the PEDF Ievel in eyes with NPDR [(7.00 ± 2.04) ng/ml, (79.80 ± 19.89) μV, P < 0.01] and PDR [(6.39 ± 1.66) ng/ml, (28.55 ± 17.47) μV, P < 0.01] was sig-nificantly lower than that in controls [(13.87 ± 0.36) ng/ml, (237.96 ± 35.96) μV]; the concentration of PEDF in the patients with type 2 diabetes mellitus was significantly lower than that in the NPDR and PDR group (P = 0.04, P = 0.02), while there was no significant difference between NPDR group and PDR group (P = 0.34). There were significant differences in different OPs group (P < 0.01). The PEDF concentration in the aqueous humor of the diabetic retinopathy was a typical positive correlation with the total amplitude of OPs (r = 0.905, P < 0.01). Conclusion: The concentration of PEDF in aqueous humor and the total amplitude of OPs were positively correlated with the diabetic retinopathy, which could provide an objective basis for the early diagnosis of diabetic retinopathy and the prediction of the progression of the disease.
文章引用: 臧 晶 , 管国奇 , 王文娟 , 鲍炯琳 , 陈立伦 (2016) 各期糖尿病视网膜病变患者房水中PEDF与OPs的相关性分析。 眼科学， 5， 22-28. doi: 10.12677/HJO.2016.51005
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