PCDH10基因DNA甲基化在胃癌中的研究
Research of PCDH10 DNA Methylation in Gastric Cancer

作者: 鞠亚菲 :山东省济宁市兖州区中医医院脾胃病科,山东 济宁 ; 姜相君 :青岛市市立医院消化内科,山东 青岛;

关键词: 原钙粘蛋白10甲基化特异性聚合酶链反应实时荧光定量逆转录–多聚合酶链反应胃癌PCDH10 MSP RT-PCR Gastric Cancer

摘要:
目的:探讨PCDH10(原钙粘蛋白10)基因DNA发生甲基化后,其表达与胃癌发生的关系。方法:采用甲基化特异性PCR (methy-lation specific PCR)法和实时荧光定量逆转录–多聚合酶链反应(real-time RT-PCR)方法检测100例胃癌组织及正常组织中PCDH10基因启动子区的甲基化情况。结果:胃癌组织中PCDH10基因的甲基化阳性率较正常组织明显升高,差异具有统计学意义。结论:PCDH10的基因DNA甲基化可能与胃癌发生有关。

Abstract: Objective: To analyze and compare methylation status of PCDH10 gene in gastric cancer and nomal tissues. Methods: Methylation-Specific PCR (MSP) and RT-PCR (real-time RT-PCR) were applied to detect DNA methylation features of the PCDH10 gene in 100 smples. Results: The PCDH10 gene methylation in the gastric cancer tissues was significantly higher than that in normal tissues. Con-clusion: PCDH10 DNA methylation is correlated with gastric cancer.

文章引用: 鞠亚菲 , 姜相君 (2016) PCDH10基因DNA甲基化在胃癌中的研究。 临床医学进展, 6, 45-49. doi: 10.12677/ACM.2016.61009

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