Preparation and Characterization of Liposomes Embedded with Hydrophobic Fe3O4 Nano-Particles
Abstract: Magnetic liposomes not only have good biocompatibility but also have excellent magnetic proper-ties, so it is widely used in tumor tissue to enhance MR angiography in order to achieve prevention and early diagnosis of tumors. Here, we describe synthesis of magnetic nano-composite liposomes (HMLs) by a thin film dispersing method, based on hydrophobic magnetite (Fe3O4) nanoparticles. We studied the effect of lipid concentration, lecithin and cholesterol mass ratio for the properties of HMLs. The results showed that the size of the HMLs containing Fe3O4-OA NPs mainly in a sand-wich-structure is 125.3 ± 12.9 nm obtained by transmission electron microscopy (TEM) and dy-namic light scattering (DLS). The optimized prescription of HMLs has been obtained by orthogonal tests in which encapsulation efficiency of Fe is used as index and phenanthroline absorption spec-trophotometry is used to determine Fe content. The results showed that the best prescription pre- paration process of HMLs is: 0.5 - 2.0 mg/mL of lecithin, and [lecithin]:[cholesterol] = 2:1 - 6:1 (w/w). And while the initial Fe concentration in the solution varied from 0.25 to 3.0 mg/mL, an effective Fe3O4 NPs loading was achieved, with encapsulation efficiency (EE%) from 91.0% to 71.0%. In a word, the results affirm the HMLs possess high Fe concent and can be potentially used to enhance Magnetic Resonance Imaging (MRI) in tumor tissues.
文章引用: 韩利敏 , 潘立志 , 楚险峰 , 周兴平 (2016) 以疏水性Fe3O4纳米粒子为基的脂质体的制备及表。 物理化学进展， 5， 1-8. doi: 10.12677/JAPC.2016.51001
Mornet, S., Portier, J. and Duguet, E. (2005) A Method for Synthesis and Functionalization of Ultrasmall Superpara-magnetic Covalent Carriers Based on Maghemite and Dextran. Journal of Magnetism and Magnetic Materials, 293, 127-134.
Mornet, S., Vasseur, S., Grasset, F., et al. (2004) Magnetic Nanoparticle Design for Medical Diagnosis and Therapy. Journal of Materials Chemistry, 14, 2161-2175.
Zhang, Z., van den Bos, E.J., Wielopolski, P.A. de Jong-Popijus, M., Duncker, D.J. and Krestin, G.P. (2004) High- Resolution Magnetic Resonance Imaging of Iron-Labeled Myoblasts Using a Standard 1.5-T Clinical Scanner. Magma, 17, 201-209.
 许乙凯. 磁共振对比剂的发展概况及存在问题[J]. 南方医科大学学报, 2002, 9(9): 769-971.
 Prajapati, B.G. (2007) A Review on PEGylated Liposome in Cancer Therapy and in Delivery of Biomaterial. Pharmaceutical Reviews, 6, 153-161.
Hanuš, J., Ullrich, M., Dohnal, J., et al. (2013) Remotely Controlled Diffusion from Magnetic Liposome Microgels. Langmuir, 29, 4381-4387.
Park, S. (2005) Effects of Silver Nanoparticles on the Fluidity of Bilayer in Phospholipid Liposome. Colloids and Surfaces B: Biointerfaces, 44, 117-122.
 Frascione, D., Diwoky, C., Almer, G., et al. (2012) Ultrasmall Superparamagnetic Iron Oxide (USPIO)-Based Liposomes as Magnetic Resonance Imaging Probes. Nano-medicine, 7, 2349-2359.
Qiu, D., An, X., Chen, Z., et al. (2012) Microstructure Study of Liposomes Decorated by Hydrophobic Magnetic Nanoparticles. Chemistry and Physics of Lipids, 165, 563-570.
Hohner, A., David, M.P.C. Dlera, R. (2010) Controlled Solvent-Exchange Deposition of Phoslipid Memnranes onto Solid Surface. Biointerphases, 5, 1-8.