一株传染性法氏囊病毒野毒株的分离与鉴定
Identification and Isolation of a Wild Strain of Infectious Bursal Disease Virus

作者: 付梦姣 , 王永强 , 李晓齐 , 曹 红 , 陈福勇 , 郑世军 :中国农业大学动物医学院,北京;

关键词: 传染性法氏囊病毒VP2毒力Infectious Bursal Disease Virus VP2 Virulence

摘要:
笔者从北京延庆地区一鸡场采集发病鸡的肾脏、脾、法氏囊样品,通过鸡胚接种及RT-PCR技术初步诊断其病原为传染性法氏囊病毒,暂命名为BY-2015-1。本文设计IBDV VP2的引物,以反转录得到的cDNA为模板进行扩增,并对扩增得到的序列进行序列分析,序列分析显示该分离株的核苷酸序列与标准强毒株OKYM的同源性为97.7%,分析VP2高变区的氨基酸序列发现,此分离株在VP2高变区关键氨基酸位点的变异符合强毒株的特点,但又不完全相同。该毒株的分离和鉴定,为传染性法氏囊病防控和流行病学调查提供了依据。

Abstract: The kidney, spleen and bursa of fabricius samples were collected from diseased chickens on a farm in Beijing suburban areas. A wild strain of infectious bursal disease virus (IBDV), temporarily called BY-2015-1, was isolated by embryonic inoculation and identified via RT-PCR assay and sequence analysis. Gene sequence analysis of VP2 showed that the homology between BY-2015-1 and OKYM strains was 97.7%. The amino acid sequence analysis of VP2 high variable region indicated that BY-2015-1 isolate had similar characteristics of very virulent strains, suggesting that the isolate might be a circulating virulent strain in the flocks on the farm. The isolation and identification of IBDV BY-2015-1 strain would help to prevent and control IBDV in the local areas.

文章引用: 付梦姣 , 王永强 , 李晓齐 , 曹 红 , 陈福勇 , 郑世军 (2016) 一株传染性法氏囊病毒野毒株的分离与鉴定。 亚洲兽医病例研究, 5, 5-10. doi: 10.12677/ACRPVM.2016.51002

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