The Expression and Clinical Significance of miR-183 in Gastric Cancer of Different Tumor Stages

作者: 顾 玮 , 胡梅洁 , 胡晓莹 , 孙 颖 , 马 瑾 :上海交通大学医学院附属瑞金医院卢湾分院消化内科,上海;

关键词: 微小RNAmiR-183胃癌TNM分期micro-RNA miR-183 Gastric Cancer TNM Stage

目的:检测miR-183在不同分期胃癌中的表达量并讨论其临床意义。方法:实时定量PCR(real-time PCR,RT-PCR)法检测100例胃癌组织与其中20例相应癌旁组织中miR-183的表达水平,分析miR-183表达水平与胃癌患者临床病理特征及预后的关系。结果:胃癌组织中miR-183表达量明显高于相对应癌旁正常组织(2.12 ± 0.24比1.04 ± 0.15,p < 0.01) miRNA-183表达水平与临床病理肿瘤MTN分型,淋巴结转移,远处转移及肿瘤浸润程度有关(p < 0.05),与性别、年龄、肿瘤大小、分化程度无关(p > 0.05)。ROC曲线分析显示,miR-183在预测肿瘤转移时,其AUC值为0.74 (95% CI为0.530~0.865),敏感度为50.0%,特异性为90.0%;结论:miR-183在胃癌组织中的表达上调与胃癌的临床病理特征及患者预后密切相关,miR-183可能成为胃癌诊断及预后判断的新的分子标志。

Abstract: Objective: To quantitate expression of miR-183 in gastric cancer of different tumor stages and discuss their significances. Methods: Real-time PCR was used to detect the miR-183 expressions between gastric cancer tissues and matched adjacent tissues. The correlations of miR-183 expres-sion with clinic-pathological features and clinical prognosis were analyzed. Results: The significant overexpression of miR-183 was observed in the rectal cancer tissues (2.12 ± 0.24 versus 1.04 ± 0.15, p < 0.01) as compared with the corresponding adjacent normal tissues. Up-regulation of miR-183 is associated with advanced clinical stage, positive lymph node, deep stromal invasion, and distant metastasis in gastric cancer patients. Conclusion: The expression of miR-183 is associated with clinic-pathological features and patient’s clinical prognosis, so it may be used as a potential diagnostic biomarker and a prognostic predictor in patients with gastric cancer.

文章引用: 顾 玮 , 胡梅洁 , 胡晓莹 , 孙 颖 , 马 瑾 (2015) miR-183在不同分期胃癌中的表达及意义。 临床医学进展, 5, 219-224. doi: 10.12677/ACM.2015.54035


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