Analysis of Shikimic Acid Content in the Needle of Larix gmelinii by HPLC
Abstract: In this paper, the dynamic change of shikimic acid contents in the needle of Larix gmelinii during a year was studied using the method of HPLC. The fresh pine needle from the upper, middle and lower parts of 5 Larix gmelinii trees with the same age was collected from middle of March to middle of November in 2015. The weight per sample was 300.0 g. The samples collected in the same month were homogeneous mixing and dried in the shade. Before using, the sample was grounded and sieved into homogeneous size. The dried sample powder was mixed with methonal for ultrasonic treatment to extract shikimic acid. The extract was concentrated and re-solved for analysis of HPLC. The results showed that the shikimic acid contents in the needle of Larix gmelinii ranged from 0.04% to 0.44%, while the contents of shikimic acid in Larix gmelinii changed during different months. The highest content reached 0.44% in the middle of June, with the time increasing; the lowest content reached 0.04% in November. The paper determines the contents of shikimic acid contents in the needle of Larix gmelinii, in order to find a good substitute of raw materials for industrial production of shikimic acid.
文章引用: 高文博 , 李丽丽 , 杜新奇 , 崔国强 , 陈凤丽 (2016) HPLC法测定兴安落叶松针叶中莽草酸含量。 植物学研究， 5， 6-10. doi: 10.12677/BR.2016.51002
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