马铃薯晚疫病生防菌株的筛选及其拮抗作用的研究
The Study of Screening and Antagonism for Potato Late Blight Biocontrol

作者: 曹 旭 , 刘宇帅 , 张淑梅 , 孟利强 , 姜 威 , 李 晶 :黑龙江省科学院微生物研究所,黑龙江 哈尔滨;黑龙江省生物工程重点实验室,黑龙江 哈尔滨;黑龙江省科学院高技术研究院,黑龙江 哈尔滨; 陈静宇 , 胡基华 :黑龙江省生物工程重点实验室,黑龙江 哈尔滨;黑龙江省科学院高技术研究院,黑龙江 哈尔滨;

关键词: 马铃薯晚疫病生防菌筛选Potato Late Blight Biocontrol Fungus Screening

摘要:
目的:筛选获得对马铃薯晚疫病具有较好防治作用的生防菌株,并对其拮抗作用进行研究。方法:利用平板抑菌对峙培养法筛选生防菌,并采用梯度稀释法及共培养的方法对拮抗作用机制进行初步探索。结果:筛选出2株对马铃薯晚疫病菌具有抑制作用的生防菌株PB12、CZS;通过抑菌试验表明,菌株PB12和CZS的抑菌率可达98%,在液体培养时生防菌液稀释度的变化对抑菌效果没有明显影响,在固体培养时随着生防菌液稀释度的增加抑菌能力稍有下降。结论:筛选出2株对马铃薯晚疫病菌具有较强拮抗作用的生防菌株PB12和CZS,并通过抑制病原菌菌丝的生长达到抑菌效果。

Abstract: Aim: To screen biocontrol strains that with better control effect on potato late blight and then study the biocontrol strains antagonistic effect. Methods: Screening of biocontrol bacteria cultured by plate confrontation inhibition, the mechanism of antagonism was preliminarily explored by using gradient dilution method and co-culture method. Results: Screening 2 strains which had inhibitory effect on potato late blight pathogen biocontrol strains of PB12 and CZS, the antibacterial test showed that the inhibition rate of PB12 and CZS was 98%; and the change of biocontrol dilution degree in liquid culture had little effect on the antibacterial effect, while decreased slightly in the solid medium. Conclusion: We screen 2 strains with strong antagonistic effect on potato late blight pathogen biocontrol strains of PB12 and CZS, and achieve the antibacterial effect by inhibiting the growth of pathogenic bacteria.

文章引用: 曹 旭 , 刘宇帅 , 陈静宇 , 张淑梅 , 孟利强 , 胡基华 , 姜 威 , 李 晶 (2015) 马铃薯晚疫病生防菌株的筛选及其拮抗作用的研究。 微生物前沿, 4, 55-61. doi: 10.12677/AMB.2015.44008

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