Effects of Carotenoids on 3T3-L1 Preadipocyte Viability and Differentiation
Abstract: The aim of the study was to assess the suppressive effects of bixin, β-carotene and lycopene on the differentiation of 3T3-L1 preadipocytes. The viability of 3T3-L1 cells were tested by MTT and their differentiation was evaluated by Oil red O staining method. The expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) were measured by West blotting. The results showed that bixin, β-carotene and lycopene significantly reduced the viability and suppressed the differentiation of 3T3-L1 preadipocytes, respectively. β-Carotene had stronger suppressive effects than bixin and lycopene on adipocyte differentiation in 3T3-L1 cells. Bixin, β-carotene and lycopene down-regulated PPARγ expression. These results suggest that bixin, β-carotene and lycopene inhibit the adipocyte differentiation of 3T3-L1 cells through down-regulation of PPARγ.
文章引用: 高润草 , 阮宝玉 , 吕 品 , 赵文恩 (2015) 类胡萝卜素对前脂肪细胞3T3-L1增殖和分化的影响。 生物物理学， 3， 49-57. doi: 10.12677/BIPHY.2015.32005
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