The Study of Extraction and Purification of Lactones from Bamboo Leaves
Abstract: In this article, the extraction and purification of the lactones from the bamboo leaves were studied. First, after pretreatment of bamboo leaves, the lactones were extracted with ethanol in terms of the extraction rate of lactones. Then, the optimal parameters, such as concentration of enthanol, solid-liquid ratio, temperature and time of extraction were determined in single factor experiments. Finally, the best extraction condition was optimized in an orthogonal experiment with four factors and three levels (L9(34)). The results indicated that the optimal conditions were 40% of ethanol concentration, liquid-solid ratio 15:1, extraction temperature at 90˚C and extraction duration of 3 h. After centrifugation of the extract, the supernatant was concentrated and then subjected to purification by polyamide resins, DM301, D101, HP-20 and AB-8, respectively. Taken the static adsorption and desorption rate as evaluation indexes, the result showed that polyamide had better adsorption and desorption rate for bamboo lactone than the others, which was also favorable for the separation of lactones and flavonoids from bamboo leaves. Therefore, polyamide was chosen as the best resin. Subsequently, after concentration, the extract was subjected to the column separation, followed by elution with 20% ethanol before the effluent was collected. Finally, after lyophilization and concentration in turn, the extract was collected, in which the yield (according to the original bamboo leaves) and purity of lactones in the extract were 9.44‰ and 46.2%, respectively.
文章引用: 章萍萍 , 孙汉巨 , 娄秋艳 , 王 鑫 , 谢玉鹏 , 王 霄 , 张曼莉 (2015) 竹叶内酯的提取及纯化工艺研究。 食品与营养科学， 4， 76-86. doi: 10.12677/HJFNS.2015.43011
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