In Vivo Screening with Ph.D.-7TM and Identification of Ovarian Cancer A2780 Cell Targeted Heptapeptides
Abstract: In order to select the specific ligands for ovarian cancer, the in vivo phage display technology has been applied to screen the peptide targeting to ovarian cancer A2780 cells. Firstly, ovarian cancer A2780 cells were inoculated into the armpit of nude mice to establish a tumor-bearing animal model. Then in vivo biopanning was conducted for 3 rounds with the Ph.D.-7TM phage-displayed peptide library, and individual phage clones were picked for DNA sequencing after the 3rd round. Among the picked phage clones, the clone PC-H displaying heptapeptide HGGVRLY (peptide HGG) accounted for as high as 27 percent. The identification results by ELISA tests, in vivo and in vitro phage binding assays, competitive binding assays, and immunofluorescence assays indicated that both the phage clone PC-H and the peptide HGG could be preferentially bounded to A2780 cells and then concentrated in tumor tissues. Furthermore, the results of MTT assay and scratch assay revealed that peptide HGG possessed low cytotoxicity. Altogether, the peptide HGG screened with the Ph.D.-7TM exhibited the high affinity to A2780 cells and the targeting to the ovarian cancer tis-sues, and would have great potentials in the targeting drug delivery system for ovarian cancer.
文章引用: 李钰璨 , 尹光福 , 蒲曦鸣 , 尤 飞 (2015) Ph.D.-7TM肽库体内淘选卵巢癌A2780细胞特异性结合肽。 世界肿瘤研究， 5， 43-51. doi: 10.12677/WJCR.2015.53007
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