The Expression of p21WAF1/CIP1in Rabbit Proliferative Vitreoretinopathy
Abstract: Objective: To observe the expression of p21WAF1/CIP1 during the course of experimental proliferative vitreoretinopathy (PVR) in rabbits and analyze its possible mechanism. Method: Thirty- one pigmented rabbits were divided into two groups, experimental group in 28 rabbits and normal control group in 3 rabbits. Histology was used to observe PVR progress. Western blot and reverse transcription-polymerase chain reaction (RT-PCR) methods were used to detect the expression of p21WAF1/CIP1, CDK2, cyclin E protein or mRNA in retina on different set points. Results: Inflammatory cells infiltration was observed on day 7 in experimental rabbits’ retina. The proliferative typical strip of local retina was formatted on day 14 and fixed fold was noticed on the retina on day 28. Western blot method showed that the relative expression of p21WAF1/CIP1 protein was lowest on day 14, and the relative expression of CDK2 and cyclin E protein were highest on day 14. The difference with that in other set points had strong statistic significance (F = 20.55, P = 0.00; F = 26.903, P = 0.000; F = 10.00, P = 0.00). RT-PCR method also showed that the lowest relative expression on the relative expression of p21WAF1/CIP1 mRNA was on day 14, and the highest relative expression on the relative expression of CDK2 and cyclin E mRNA were noticed on day 14. The difference had strong statistic significance with that in other groups (F = 18.06, P = 0.00; F = 14.90, P = 0.00; F = 87.64, P = 0.00). Conclusions: The expression of p21WAF1/CIP1 decreased during the course of PVR, which might contribute to the progress of PVR by regulation CDK2 and cyclin E levels.
文章引用: 由彩云 , 袁志刚 , 曾 峥 , 颜 华 (2015) p21WAF1/CIP1在兔增生性玻璃体视网膜病变视网膜中的表达。 眼科学， 4， 27-33. doi: 10.12677/HJO.2015.42005
 Iandiev, I., Bringmann, A. and Wiedemann, P. (2010) Proliferative vitreoretinopathy pathogenesis and therapy. Klin Monbl Augenheilkd, 227,168-174.
 Jung, Y.S., Qian, Y. and Chen, X. (2010) Examination of the expanding path-ways for the regulation of p21 expression and activity. Cell Signal, 22, 1003-1012.
 袁志刚, 韩金栋, 颜华, 李海燕 (2011) 玻璃体腔注射人PRP联合巩膜外冷冻建立PVR动物模型的研究. 眼科新进展, 31, 930-933.
 Csikász-Nagy, A., Palmisano, A. and Zámborszky, J. (2011) Molecular network dynamics of cell cycle control: Transitions to start and finish. Methods in Molecular Biology, 761, 277-291.
 Blagosklonny, M.V. and Pardee, A.B. (2002) Therestriction point of the cell cycle. Cell Cycle, 1, 103-110.
 Neganova, I., Zhang, X., Atkinson, S. and Lako, M. (2009) Expression and functional analysis of G1 to S regulatory components reveals an im-portant role for CDK2 in cell cycle regulation in human embryonic stem cells. Oncogene, 28, 20-30.
 Pang, L.Y., Scott, M., Hayward, R.L., Mohammed, H., Whitelaw, C.B., Smith, G.C. and Hupp, T.R. (2011) p21 (WAF1) is com-ponent of a positive feedback loop that maintains the p53 transcriptional program. Cell Cycle, 10, 932-950.
 Lee, J.H., Lee, S.Y., Lee, J.H. and Lee, S.H. (2010) p21 WAF1 is involved in interferon-β-induced attenuation of telomerase activity and human telomerase reverse transcriptase (hTERT) expression in ovarian cancer. Molecules and Cells, 30, 327-333.
 Ma, H., Zhou, Z., Wei, S. and Wei, Q. (2011) Association between p21 Ser31Arg polymorphism and cancer risk: A meta-analysis. Chiniese Journal of Cancer, 30, 254-263.
 Nakamura, S., Kamakura, T. and Ookura, T. (2009) Tongue epithelial KT-1 cell-cycle arrest by TGF-beta associated with induction of p21(Cip1) and p15 (Ink4b). Cytotechnology, 61, 109-116.
 Abbas, T., Sivaprasad, M., Terai, K., Amador, V., Pagano, M. and Dutta, A. (2008) PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex. Genes Development, 22, 2496-2506.
 Faria, M.H., Patrocinio, R.M., Moraes Filho, M.O. and Rabenhorst, S.H. (2007) Immunoexpression of tumor suppressor genes p53, p21WAF1/CIP1 and p27KIP1 in humam astrocystic tumors. Arq Neuropsiquiatr, 65, 1114-1122.
 赵成, 游志鹏 (2009) p21WAF1/CIP1在视网膜母细胞瘤中的表达及意义. 眼科研究, 27, 903-905.
 马志中 (2010) 我国眼外伤近五年研究进展. 中华眼科杂志, 46, 911-914.
 赵霞, 马志中 (2009) 关于玻璃体手术治疗眼外伤的争议. 中国实用眼科杂志, 27, 1329-1332.