Xuebijing Regulate the Hypothalamic-Pituitary-Adrenal Axis and the Level of GRmRNA of Adrenal in Septic Rats
Abstract: Objective: To investigate the change of CORT and the level of GRmRNA of adrenal in CLP induced septic rats which were treated by Xuebijing, identify that Xuebijing can regulate the CORT and GRmRNA. Methods: 40 male rats were divided randomly into normal control group, sham group, saline group (Sepsis group), Xuebijing for treatment group. The model of sepsis was made by CLP. We observe the clinical manifestation of animal after CLP. We use the radio-immunity method to detect the levels of CORT; using the RT-PCR method to measure the level of GRmRNA in adrenal. Results: In Sepsis group, the plasma CORT concentration (29.74 ± 1.65 ng/ml) was significantly higher than that in the normal control group (7.56 ± 0.89 ng/ml, P < 0.01) and Sham group (13.27 ± 1.31 ng/ml, P < 0.01). In XBJ group, plasma CORT concentration (19.27 ± 1.85 ng/ml) was significantly lower than that in Sepsis group (P < 0.01). There was no significant difference between Normal control and Sham group (P > 0.05). In Sepsis group, the level of the GRmRNA in the adrenal tissue (0.80 ± 0.11) was significantly lower than that in the normal control group (1.44 ± 0.07, P < 0.01) and the Sham group (1.15 ± 0.11, P < 0.01). There was no significant difference between XBJ (1.08 ± 0.10) and Sepsis group (P > 0.05). Conclusion: In septic rats, the HPA axis was excessive activate. Xuebijing can decrease the level of CORT, increase the level of GRmRNA in adrenal, improve the excessive activate condition of HPA axis, and lessen the clinical manifestation.
文章引用: 李宏山 , 刘淑正 , 周洁平 , 常玉坤 , 王静怡 (2015) 血必净对脓毒症大鼠皮质酮及肾上腺组织GRmRNA水平的影响。 临床医学进展， 5， 112-117. doi: 10.12677/ACM.2015.52020
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