Selectively Tumor Targeted Fe3O4 Magnetic Nanoparticles Modified with Peptide
Abstract: Fe3O4 magnetic nanoparticles (MNPs) have been widely used in tumor imaging and therapy. How-ever, low therapeutic concentration at tumor sites is one of the most important factors that limit their applications. MNPs conjugated with specific targeting ligands might selectively bind to specific tumor cells to increase the concentration of MNPs at tumor sites while the total dose decreased. Objective: Fe3O4 MNPs specific binding peptide (TVNFKLY) and ovarian tumor cells A2780 specific binding peptide (QQTNWSL) were conjugated together to form a bi-functional peptide, which was used to modify Fe3O4 MNPs, and the cytotoxicity and targeting ability of MNPs were investigated. Methods: Fourier transform infrared spectrometry, thermal analysis and fluorescence microscopy have been used to demonstrate the presence of peptide on the surface of Fe3O4 MNPs. MTT assays were employed to detect the cell viability. The targeting ability of Fe3O4 MNPs was verified by Prussian blue staining. Results: Synthesized peptide (QQTNWSLTVNFKLY) could bind to Fe3O4 MNPs, and Fe3O4 MNPs binding with peptide had no significant cytotoxicity to L929 cells and exhibited good targeting ability to tumor cells. Conclusion: The synthesized bi-functional peptide could bind to Fe3O4 MNPs, and the MNPs had better capability to target tumor cells.
文章引用: 尤 飞 , 尹光福 , 蒲曦鸣 (2015) 多肽修饰Fe3O4磁性纳米颗粒及其肿瘤细胞靶向性。 材料科学， 5， 111-118. doi: 10.12677/MS.2015.53016
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