Identification and Genetic Diversity of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Chongqing
Abstract: The visceral organs were collected from swines at a farm in ChongQing. Once the paghogen was idetified as the porcine reproductive and respiratory syndrome virus via RT-PCR identification, we used reverse transcription to make cDNA. Both NSP2 and GP5 gene were amplified by PCR, and then they were sequenced. The NSP2 sequence analysis indicated that homology between CQ and classical VR-2332 strain was 66.7%, while it was as high as 98.8% compared with HP-PRRSV vari-ation SD and JXA1 in nucleotide. And the GP5 sequence analysis indicated that there were varia-tions in individual bases. It was suggested that the CQ strain was a HP-PRRSV variation. At the same time, the patho-material was inoculated in Marc-145 cells, and then the CPE appeared. The viral titer is 10−5.67 TCID50/0.1mL by Reed-Muench. The separation of CQ strain offers basis for ep-idemiology investigations of PRRSV.
文章引用: 王 璐 , 肖 红 , 陈脊宇 , 顾 盼 , 周康森 , 王玲玲 , 黄 倩 , 黎晓敏 , 蔡家利 (2015) 高致病性猪蓝耳病病毒重庆株的分离鉴定及其变异性分析。 亚洲兽医病例研究， 4， 7-13. doi: 10.12677/ACRPVM.2015.42002
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